Human MAPK7 / ERK5
MAPK7 | Big MAP kinase 1 | BMK-1 | Erk4 | ERK5 | MAPK 7 | PRKM7 | BMK1 | BMK1 kinase | ERK-5 | MAP kinase 7
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Synthetic peptide corresponding to amino acids 213-224 of human BMK1/Erk5 (HQYFM[pT]E[pY]VATR), KLH-conjugated. The immunizing sequence is identical in mouse, 9/12aa identical in rat.
Recognizes human phospho-BMK1/Erk5 (Thr218, Tyr220), Mr ~110kD. Predicted to cross-react with mouse and rat based on sequence homology.
Suitable for use in Western Blot. Western Blot: 1-:250-1:500 detects phospho-BMK1/Erk5 (Thr218, Tyr220) in RIPA lysates from HeLa cells treated with sorbitol. Lysates from untreated or sorbitol-treated HeLa cells were resolved by electrophoresis, transferred to nitrocellulose and probed with either anti-phospho-BMK1/Erk5 (Thr218, Tyr220) (1:500) or anti-BMK1/Erk5 (1:1000). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system (BMK1/Erk5 or phospho- BMK1/Erk5 (110kD).
100 mM Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide, 30% glycerol.
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
For research use only. Intended for use by laboratory professionals.
This antibody carries the LSBio 100% Guarantee.