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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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MAPK7 / ERK5 Antibody (phospho‑Thr218/Tyr220) LS‑C15060

Antibody

MAPK7 / ERK5 Antibody (phospho‑Thr218/Tyr220) LS‑C15060

Antibody:
Sheep Polyclonal to Human MAPK7 / ERK5
Application:
WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$665 
LS-C15060-200
Toll Free North America
866-819-4732

Overview

Antibody:
Sheep Polyclonal to Human MAPK7 / ERK5
Application:
WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
ERK5 antibody LS-C15060 is an unconjugated sheep polyclonal antibody to human ERK5 (MAPK7). Validated for WB.
Target
Human MAPK7 / ERK5
Host
Sheep
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Failed Applications
  • IHC - Paraffin
Immunogen
MAPK7 / ERK5 antibody was raised against synthetic peptide corresponding to amino acids 213-224 of human BMK1/Erk5 (HQYFM[pT]E[pY]VATR), KLH-conjugated. The immunizing sequence is identical in mouse, 9/12aa identical in rat.
Epitope
pThr218/Tyr220
Specificity
Recognizes human phospho-BMK1/Erk5 (Thr218, Tyr220), Mr ~110kD. Predicted to cross-react with mouse and rat based on sequence homology.
Usage
Suitable for use in Western Blot. Western Blot: 1-:250-1:500 detects phospho-BMK1/Erk5 (Thr218, Tyr220) in RIPA lysates from HeLa cells treated with sorbitol. Lysates from untreated or sorbitol-treated HeLa cells were resolved by electrophoresis, transferred to nitrocellulose and probed with either anti-phospho-BMK1/Erk5 (Thr218, Tyr220) (1:500) or anti-BMK1/Erk5 (1:1000). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system (BMK1/Erk5 or phospho- BMK1/Erk5 (110kD).
Presentation
100 mM Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide, 30% glycerol.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MAPK7 / ERK5
Q13164 NM_002749 NP_002740.2

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Requested From: United States
Date Requested: 7/22/2019
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