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| Catalog Number | Size | Price |
|---|---|---|
| LS-C16353-200 | 200 µl | $694 |
Monoclonal Mouse anti‑Human MAPK3 / ERK1 Antibody (phospho‑Thr202/Tyr204, IF, WB) LS‑C16353
Monoclonal Mouse anti‑Human MAPK3 / ERK1 Antibody (phospho‑Thr202/Tyr204, IF, WB) LS‑C16353
Antibody:
MAPK3 / ERK1 Mouse anti-Human Monoclonal (pThr202/Tyr204) Antibody
Application:
ICC, IF, WB, IP, Flo, ELISA
Reactivity:
Human, Mouse, Rat, Hamster
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
MAPK3 / ERK1 Mouse anti-Human Monoclonal (pThr202/Tyr204) Antibody
Application:
ICC, IF, WB, IP, Flo, ELISA
Reactivity:
Human, Mouse, Rat, Hamster
Format:
Unconjugated, Unmodified
Specifications
Description
ERK1 antibody LS-C16353 is an unconjugated mouse monoclonal antibody to ERK1 (MAPK3) (pThr202/Tyr204) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, Flow, ICC, IF, IP and WB.
Target
Human MAPK3 / ERK1
Synonyms
MAPK3 | ERK-1 | ERT2 | HUMKER1A | HS44KDAP | MAP kinase 1 | MAP kinase isoform p44 | MAPK 1 | MAPK 3 | p44 | p44MAPK | p44-MAPK | p44ERK1 | MAP kinase 3 | p44-ERK1 | ERK1 | PRKM3
Host
Mouse
Reactivity
Human, Mouse, Rat, Hamster
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Immunogen
Synthetic phosphopeptide (KLH coupled) corresponding to residues around Thr202/Tyr204 of human p44 MAP kinase.
Epitope
pThr202/Tyr204
Specificity
Detects endogenous levels of human p44 and p42 MAP kinase (Erk1 and Erk2) dually phosphorylated at threonine 202 and tyrosine 204. Does not cross-react with the corresponding phosphorylated residues of either SAPK/JNK or p38 MAP kinase. Species cross-reactivity: Hamster, mouse, rat and zebrafish.
Applications
- ICC (1:200)
- Immunofluorescence (1:200)
- Western blot (1:2000)
- Immunoprecipitation (1:500)
- Flow Cytometry (1:1000)
- ELISA
Usage
Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunofluorescence and Immunocytochemistry. Western Blot: 1:2000, incubate membrane with diluted antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:500. Immunofluorescence: 1:200. Immunocytochemistry: 1:200. Flow Cytometry: 1:1000.
Presentation
10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 0.02% Sodium Azide, 50% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About MAPK3 / ERK1
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Species:
Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig
Applications:
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Applications:
ICC, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry, ELISA
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Human, Monkey
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Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig
Applications:
IHC, IHC - Paraffin
Species:
Rat, Mouse, Hamster, Rabbit
Applications:
IHC, Western blot, Immunoprecipitation
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/18/2024
Date Requested: 4/18/2024
