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Catalog Number	Size	Price
LS-C757526-200	200 µl	$393

MAPK3 / ERK1 Antibody - MAPK3/1 Antibody (Center) staining MAPK3/1 in human breast carcinoma sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

MAPK3 / ERK1 Antibody - Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling MAPK3/1 with MAPK3/1 Antibody (Center) at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG (NH174309) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (OI17558410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).

MAPK3 / ERK1 Antibody - All lanes: Anti-MAPK3/1 Antibody (Center) at 1:2000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: mouse brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 43 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

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Monoclonal Mouse anti‑Human MAPK3 / ERK1 Antibody (clone 1129CT3.4.3, Internal, IHC, IF, WB) LS‑C757526

Name: Anti-MAPK3 / ERK1 Antibody | Mouse anti-Human Monoclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C757526-200
Price: 393.0000 USD
Availability: InStock

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Antibody:

MAPK3 / ERK1 Mouse anti-Human Monoclonal (Internal) (1129CT3.4.3) Antibody

Application:

IHC-P, IF, WB, ELISA

Reactivity:

Human, Mouse

Format:

Unconjugated, Unmodified

$393

LS-C757526-200

Toll Free North America

206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

MAPK3 / ERK1 Mouse anti-Human Monoclonal (Internal) (1129CT3.4.3) Antibody

Application:

IHC-P, IF, WB, ELISA

Reactivity:

Human, Mouse

Format:

Unconjugated, Unmodified

Specifications

Description

ERK1 antibody LS-C757526 is an unconjugated mouse monoclonal antibody to ERK1 (MAPK3) (Internal) from human. It is reactive with human and mouse. Validated for ELISA, IF, IHC and WB.

Target

Human MAPK3 / ERK1

Synonyms

See All MAPK3 / ERK1 Antibodies

Host

Mouse

Reactivity

Human, Mouse (tested or 100% immunogen sequence identity)

Predicted

Rat, Bovine, Xenopus, Drosophila (at least 90% immunogen sequence identity)

Clonality

IgG1,k Monoclonal

Clone

1129CT3.4.3

Conjugations

Unconjugated

Purification

Protein G affinity chromatography

Modifications

Unmodified

Immunogen

This MAPK3/1 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 175-208 amino acids from the Central region of human MAPK3/1.

Epitope

Internal

Applications

IHC - Paraffin (1:25)
Immunofluorescence (1:25)
Western blot (1:2000)
ELISA

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

Western Blot Predicted Molecular Weight: 43.1 kDa

Presentation

PBS, 0.09% Sodium Azide

Storage

Short Term: Store at 2-8°C for up to 2 weeks. Long Term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About MAPK3 / ERK1