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Catalog Number Size Price
LS-C745386-25 25 µl (1 mg/ml) $275 
MAPK3 / ERK1 Antibody - Western Blot of rabbit Anti-ERK1 C-Term Antibody. Lane 1:MW Ladder (Opal Pre-Stained). Lane 2:HEK293 WCL. Lane 3: HeLa WCL. Lane 4: MCF-7 WCL. Lane 5: Jurkat WCL. Lane 6: A431 WCL. Lane 7: A549 WCL. Lane 8: LNCaP WCL. Lane 9: MOLT-4 WCL. Lane 10: Ramos WCL. Lane 11: Raji WCL. Lane 12: A-172 WCL. Lane 13: NIH/3T3 WCL. Lane 14: MW Ladder. Load: 10ng. Blocking: 1% Casein/TTBS buffer. Primary Antibody: ERK1 at 1µg/mL in 1% Casein/TTBS overnight at 4°C. Secondary Antibody: Goat anti-Rabbit HRP 1:30,000 in 1% Casein/TTBS for 1 hr at RT. Expect: ~44kDa.
MAPK3 / ERK1 Antibody - Western Blot of Anti-ERK1 C-Term Antibody. Lane 1: MW Marker. Lane 2: ERK1 lysate. Loaded: 10ng. Blocking: Universal Blocking Buffer MB-073 for 30 minutes at RT. Primary Antibody: Anti-ERK1 C-Term at 1:500 in MB-073 overnight at 4°C. Secondary Antibody: Goat Anti-Rabbit HRP at 1:40,000 in MB-073 for 30 min at RT. Expect: ~41 kDa.
MAPK3 / ERK1 Antibody - Western Blot of Anti-ERK1 C Term Antibody. Lane 1: MW. Lane 2: ERK1 lysate. Lane 3: ERK2 lysate. Lane 4: Mouse Brain Whole cell Lysate. Lane 5: HEK293 Whole Cell Lysate. Load: 10ng. Primary Antibody: Anti-ERK1 at 1µg/mL overnight at 4°C. Secondary Antibody: Goat anti-Rabbit Peroxidase Conjugated Antibody 1:40,000 for 30 min at RT. Blocking: BlockOut Universal Blocking buffer MB-073. Predicted MW: 43kDa in Lane 1.
MAPK3 / ERK1 Antibody - Western Blot of rabbit Anti-ERK1 C-Term Antibody. Lane 1:MW Ladder (Opal Pre-Stained). Lane 2:HEK293 WCL. Lane 3: HeLa WCL. Lane 4: MCF-7 WCL. Lane 5: Jurkat WCL. Lane 6: A431 WCL. Lane 7: A549 WCL. Lane 8: LNCaP WCL. Lane 9: MOLT-4 WCL. Lane 10: Ramos WCL. Lane 11: Raji WCL. Lane 12: A-172 WCL. Lane 13: NIH/3T3 WCL. Lane 14: MW Ladder. Load: 10ng. Blocking: 1% Casein/TTBS buffer. Primary Antibody: ERK1 at 1µg/mL in 1% Casein/TTBS overnight at 4°C. Secondary Antibody: Goat anti-Rabbit HRP 1:30,000 in 1% Casein/TTBS for 1 hr at RT. Expect: ~44kDa.
MAPK3 / ERK1 Antibody - Western Blot of Anti-ERK1 C-Term Antibody. Lane 1: MW Marker. Lane 2: ERK1 lysate. Loaded: 10ng. Blocking: Universal Blocking Buffer MB-073 for 30 minutes at RT. Primary Antibody: Anti-ERK1 C-Term at 1:500 in MB-073 overnight at 4°C. Secondary Antibody: Goat Anti-Rabbit HRP at 1:40,000 in MB-073 for 30 min at RT. Expect: ~41 kDa.
MAPK3 / ERK1 Antibody - Western Blot of Anti-ERK1 C Term Antibody. Lane 1: MW. Lane 2: ERK1 lysate. Lane 3: ERK2 lysate. Lane 4: Mouse Brain Whole cell Lysate. Lane 5: HEK293 Whole Cell Lysate. Load: 10ng. Primary Antibody: Anti-ERK1 at 1µg/mL overnight at 4°C. Secondary Antibody: Goat anti-Rabbit Peroxidase Conjugated Antibody 1:40,000 for 30 min at RT. Blocking: BlockOut Universal Blocking buffer MB-073. Predicted MW: 43kDa in Lane 1.
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Polyclonal Rabbit anti‑Human MAPK3 / ERK1 Antibody (C‑Terminus, WB) LS‑C745386

Polyclonal Rabbit anti‑Human MAPK3 / ERK1 Antibody (C‑Terminus, WB) LS‑C745386

Antibody:
MAPK3 / ERK1 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C745386-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
MAPK3 / ERK1 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
ERK1 antibody LS-C745386 is an unconjugated rabbit polyclonal antibody to ERK1 (MAPK3) (C-Terminus) from human. It is reactive with human, mouse and rat. Validated for ELISA and WB.
Target
Human MAPK3 / ERK1
Synonyms
MAPK3 | ERK-1 | ERT2 | HUMKER1A | HS44KDAP | MAP kinase 1 | MAP kinase isoform p44 | MAPK 1 | MAPK 3 | p44 | p44MAPK | p44-MAPK | p44ERK1 | MAP kinase 3 | p44-ERK1 | ERK1 | PRKM3
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Predicted
Human, Mouse, Rat (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Anti-ERK1 Antibody was produced in rabbits by repeated immunizations with synthetic peptide corresponding to amino acid residues near the C-Terminus conjugated to KLH.
Epitope
C-Terminus
Specificity
Cross reactivity is expected to occur with human, mouse and rat based on sequence identity of the peptide immunogen. This antibody does not react with the ERK2 isoform.
Applications
  • Western blot (1 µg/ml)
  • ELISA (1:40000)
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MAPK3 / ERK1
P27361 X60188 CAA42744.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/11/2021