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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MAPK12 / ERK6 / SAPK3 Antibody (clone 2F1) LS-C172516

Anti-MAPK7 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPK7.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK7.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MAPK7 antibody, and then analyzed by flow cytometry.
Anti-MAPK7 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPK7.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK7.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MAPK7 antibody, and then analyzed by flow cytometry.
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MAPK12 / ERK6 / SAPK3 Antibody (clone 2F1) LS-C172516

Antibody:
Mouse Monoclonal to Human MAPK12 / ERK6 / SAPK3
Application:
IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$345 
LS-C172516-100
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human MAPK12 / ERK6 / SAPK3
Application:
IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
SAPK3 antibody LS-C172516 is an unconjugated mouse monoclonal antibody to human SAPK3 (MAPK12 / ERK6). Validated for Flow, IF and WB.

Target
Human MAPK12 / ERK6 / SAPK3
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
2F1
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • Immunofluorescence (1:100)
  • Western blot (1:2000)
  • Flow Cytometry (1:100)
Immunogen
Full length human recombinant protein of human MAPK7 (NP_002740) produced in HEK293T cell.
Specificity
Human MAPK12 / ERK6
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MAPK12 / ERK6 / SAPK3
P53778 NM_002969 NP_002960.2

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Reactivity: Mouse
Range: 0.625-40 ng/ml
Mixed neuron/glial cultures. The perikarya and dendrites of neurons are strongly and specifically stained with the MAP2 antibody (red). Cell nuclei are visualized with DAPI DNA stain.
Species: Bovine, Human, Mouse, Rat
Applications: Immunofluorescence, Western blot
Reactivity: Mouse
Range: 31.2-2000 pg/ml

Requested From: United States
Date Requested: 5/26/2019
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