Products
Research Areas
COVID-19
Resources
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us

Locations


Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464


Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers



Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

Contact Us



Additional Contact Details

Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C744860-25 25 µl (1 mg/ml) $304 
MAPK1 / ERK2 Antibody - ELISA results of purified Mouse Anti-ERK2 (internal) Monoclonal Antibody tested against BSA-conjugated peptide of immunizing peptide ERK2 internal sequence (green line), as well as ERK2 c-term, ERK1 c-term and ERK1 n-term. Each well was coated in duplicate with 0.1µg of conjugate. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer MB-060-1000, Rb-Anti-Ms IgG HRP conjugate, and TMB-1000 substrate.
MAPK1 / ERK2 Antibody - Western Blot of Mouse anti-ERK2 Antibody. Lane 1: Opal Pre-Stained Molecular Weight Ladder. Lane 2: ERK1 50ng. Lane 3: ERK2 50ng. Primary Antibody: Anti-ERK2 (internal) Monoclonal Antibody at 1µg/mL. Secondary Antibody: Rabbit anti-Mouse IgG HRP at 1:40,000. Blocking Buffer: BlockOut MB-073 for 30 min at RT. Expect: ~41kDa seen in lane 2 only.
MAPK1 / ERK2 Antibody - ELISA results of purified Mouse Anti-ERK2 (internal) Monoclonal Antibody tested against BSA-conjugated peptide of immunizing peptide ERK2 internal sequence (green line), as well as ERK2 c-term, ERK1 c-term and ERK1 n-term. Each well was coated in duplicate with 0.1µg of conjugate. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer MB-060-1000, Rb-Anti-Ms IgG HRP conjugate, and TMB-1000 substrate.
MAPK1 / ERK2 Antibody - Western Blot of Mouse anti-ERK2 Antibody. Lane 1: Opal Pre-Stained Molecular Weight Ladder. Lane 2: ERK1 50ng. Lane 3: ERK2 50ng. Primary Antibody: Anti-ERK2 (internal) Monoclonal Antibody at 1µg/mL. Secondary Antibody: Rabbit anti-Mouse IgG HRP at 1:40,000. Blocking Buffer: BlockOut MB-073 for 30 min at RT. Expect: ~41kDa seen in lane 2 only.
1 of 2
2 of 2

Monoclonal Mouse anti‑Human MAPK1 / ERK2 Antibody (clone 3H9.A1.A8, Internal, WB) LS‑C744860

Monoclonal Mouse anti‑Human MAPK1 / ERK2 Antibody (clone 3H9.A1.A8, Internal, WB) LS‑C744860

Antibody:
MAPK1 / ERK2 Mouse anti-Human Monoclonal (Internal) (3H9.A1.A8) Antibody
Application:
WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$304
LS-C744860-25
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
MAPK1 / ERK2 Mouse anti-Human Monoclonal (Internal) (3H9.A1.A8) Antibody
Application:
WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
ERK2 antibody LS-C744860 is an unconjugated mouse monoclonal antibody to ERK2 (MAPK1) (Internal) from human. It is reactive with human, mouse and rat. Validated for ELISA and WB.
Target
Human MAPK1 / ERK2
Synonyms
MAPK1 | ERK | ERK-2 | ERK2 | MAP kinase 1 | MAPK 2 | MAPK2 | MAP kinase isoform p42 | MAPK 1 | p41mapk | PRKM2 | MAP kinase 2 | p41 | p42-MAPK | PRKM1 | ERT1 | p38 | p40 | p42MAPK | Protein tyrosine kinase ERK2
Host
Mouse
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Monoclonal
Clone
3H9.A1.A8
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Anti-ERK2 Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near an internal region of ERK2 conjugated to KLH.
Epitope
Internal
Applications
  • Western blot (1 µg/ml)
  • ELISA (1:40000)
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MAPK1 / ERK2
P28482 NM_002745 NP_002736.3

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/18/2024