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Catalog Number Size Price
LS-C744860-25 25 µl (1 mg/ml) $275 
MAPK1 / ERK2 Antibody - ELISA results of purified Mouse Anti-ERK2 (internal) Monoclonal Antibody tested against BSA-conjugated peptide of immunizing peptide ERK2 internal sequence (green line), as well as ERK2 c-term, ERK1 c-term and ERK1 n-term. Each well was coated in duplicate with 0.1µg of conjugate. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer MB-060-1000, Rb-Anti-Ms IgG HRP conjugate, and TMB-1000 substrate.
MAPK1 / ERK2 Antibody - Western Blot of Mouse anti-ERK2 Antibody. Lane 1: Opal Pre-Stained Molecular Weight Ladder. Lane 2: ERK1 50ng. Lane 3: ERK2 50ng. Primary Antibody: Anti-ERK2 (internal) Monoclonal Antibody at 1µg/mL. Secondary Antibody: Rabbit anti-Mouse IgG HRP at 1:40,000. Blocking Buffer: BlockOut MB-073 for 30 min at RT. Expect: ~41kDa seen in lane 2 only.
MAPK1 / ERK2 Antibody - ELISA results of purified Mouse Anti-ERK2 (internal) Monoclonal Antibody tested against BSA-conjugated peptide of immunizing peptide ERK2 internal sequence (green line), as well as ERK2 c-term, ERK1 c-term and ERK1 n-term. Each well was coated in duplicate with 0.1µg of conjugate. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer MB-060-1000, Rb-Anti-Ms IgG HRP conjugate, and TMB-1000 substrate.
MAPK1 / ERK2 Antibody - Western Blot of Mouse anti-ERK2 Antibody. Lane 1: Opal Pre-Stained Molecular Weight Ladder. Lane 2: ERK1 50ng. Lane 3: ERK2 50ng. Primary Antibody: Anti-ERK2 (internal) Monoclonal Antibody at 1µg/mL. Secondary Antibody: Rabbit anti-Mouse IgG HRP at 1:40,000. Blocking Buffer: BlockOut MB-073 for 30 min at RT. Expect: ~41kDa seen in lane 2 only.
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Monoclonal Mouse anti‑Human MAPK1 / ERK2 Antibody (clone 3H9.A1.A8, Internal, WB) LS‑C744860

Monoclonal Mouse anti‑Human MAPK1 / ERK2 Antibody (clone 3H9.A1.A8, Internal, WB) LS‑C744860

Antibody:
MAPK1 / ERK2 Mouse anti-Human Monoclonal (Internal) (3H9.A1.A8) Antibody
Application:
WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C744860-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
MAPK1 / ERK2 Mouse anti-Human Monoclonal (Internal) (3H9.A1.A8) Antibody
Application:
WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
ERK2 antibody LS-C744860 is an unconjugated mouse monoclonal antibody to ERK2 (MAPK1) (Internal) from human. It is reactive with human, mouse and rat. Validated for ELISA and WB.
Target
Human MAPK1 / ERK2
Synonyms
MAPK1 | ERK | ERK-2 | ERK2 | MAP kinase 1 | MAPK 2 | MAPK2 | MAP kinase isoform p42 | MAPK 1 | p41mapk | PRKM2 | MAP kinase 2 | p41 | p42-MAPK | PRKM1 | ERT1 | p38 | p40 | p42MAPK | Protein tyrosine kinase ERK2
Host
Mouse
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Monoclonal
Clone
3H9.A1.A8
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Anti-ERK2 Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near an internal region of ERK2 conjugated to KLH.
Epitope
Internal
Applications
  • Western blot (1 µg/ml)
  • ELISA (1:40000)
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MAPK1 / ERK2
P28482 NM_002745 NP_002736.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/6/2021