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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MAP2K7 / MEK7 Antibody LS-C16401

MEK7 antibody LS-C16401 is an unconjugated mouse monoclonal antibody to MEK7 (MAP2K7) from human and mouse. Validated for ELISA, IF and WB.
Catalog
Size
Price
LS-C16401-100
100 µg
$680
Description
MEK7 antibody LS-C16401 is an unconjugated mouse monoclonal antibody to MEK7 (MAP2K7) from human and mouse. Validated for ELISA, IF and WB.
Target
Human MAP2K7 / MEK7
Host
Mouse
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Immunofluorescence (15 - 20 µg/ml)
  • Western blot (1 - 3 µg/ml)
  • ELISA (0.1 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
MAP2K7 / MEK7 antibody was raised against recombinant GST-fusion protein containing full length human MKK7 sequence.
Specificity
Reacts with the ~47kD human and mouse MKK7 protein. There is no cross reactivity to MKK4. Reactivity is confirmed with MKK7 transfected COS cells, MDCK, mouse liver and Caco2 cell lysates. MDCK is the recommended positive control for this antibody.
Usage
Suitable for use in Immunofluorescence, Western Blot and ELISA. Immunofluorescence: 15-20 ug/ml. Western Blot: 1-3 ug/ml. ELISA: 0.1-1 ug/ml.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.
About MAP2K7 / MEK7
O14733 AF013589 AAC16273.1

Popular MAP2K7 / MEK7 Products

Antibody
Species: Human
Applications: Western blot
WB: Recombinant MKK7b1 (14-542) was left untreated (Lane 1) or phosphorylated by MEKK1 (14-196) (Lane 2) and probed with anti-phospho-MKK7/SKK4 (Thr275/Ser277) (1:1000 dilution).
Species: Human
Applications: Western blot
Detection of Human and Mouse MKK7 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-MKK7 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Species: Human, Mouse, Rat, Dog, Pig, Sheep, Xenopus
Applications: Western blot, Immunoprecipitation
Immunohistochemistry analysis of paraffin-embedded human brain, using MAP2K7 (Phospho-Ser271) Antibody. The picture on the right is blocked with the phospho peptide.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Mouse, Human
Applications: IHC, IHC - Paraffin, Western blot

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Requested From: United States
Date Requested: 4/21/2019
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