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Catalog Number Size Price
LS-C114531-100 100 µl (1 mg/ml) $434 
MAP2K4 / MKK4 Antibody - IHC of paraffin-embedded Ovary tissue using anti-MAP2K4 mouse monoclonal antibody. (Dilution 1:50).
MAP2K4 / MKK4 Antibody - IHC of paraffin-embedded Carcinoma of lung tissue using anti-MAP2K4 mouse monoclonal antibody. (Dilution 1:50).
MAP2K4 / MKK4 Antibody - IHC of paraffin-embedded Carcinoma of prostate tissue using anti-MAP2K4 mouse monoclonal antibody. (Dilution 1:50).
MAP2K4 / MKK4 Antibody - IHC of paraffin-embedded pancreas tissue using anti-MAP2K4 mouse monoclonal antibody. (Dilution 1:50).
MAP2K4 / MKK4 Antibody - IHC of paraffin-embedded colon tissue using anti-MAP2K4 mouse monoclonal antibody. (Dilution 1:50).
MAP2K4 / MKK4 Antibody - IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-MAP2K4 mouse monoclonal antibody. (Dilution 1:50).
MAP2K4 / MKK4 Antibody - IHC of paraffin-embedded Carcinoma of liver tissue using anti-MAP2K4 mouse monoclonal antibody. (Dilution 1:50).
MAP2K4 / MKK4 Antibody - IHC of paraffin-embedded liver tissue using anti-MAP2K4 mouse monoclonal antibody. (Dilution 1:50).
MAP2K4 / MKK4 Antibody - Anti-MAP2K4 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAP2K4.
MAP2K4 / MKK4 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP2K4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP2K4.
MAP2K4 / MKK4 Antibody - Immunoprecipitation(IP) of MAP2K4 by using monoclonal anti-MAP2K4 antibodies (Negative control: IP without adding anti-MAP2K4 antibody.). For each experiment, 500ul of DDK tagged MAP2K4 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-MAP2K4 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
MAP2K4 / MKK4 Antibody - IHC of paraffin-embedded Ovary tissue using anti-MAP2K4 mouse monoclonal antibody. (Dilution 1:50).
MAP2K4 / MKK4 Antibody - IHC of paraffin-embedded Carcinoma of lung tissue using anti-MAP2K4 mouse monoclonal antibody. (Dilution 1:50).
MAP2K4 / MKK4 Antibody - IHC of paraffin-embedded Carcinoma of prostate tissue using anti-MAP2K4 mouse monoclonal antibody. (Dilution 1:50).
MAP2K4 / MKK4 Antibody - IHC of paraffin-embedded pancreas tissue using anti-MAP2K4 mouse monoclonal antibody. (Dilution 1:50).
MAP2K4 / MKK4 Antibody - IHC of paraffin-embedded colon tissue using anti-MAP2K4 mouse monoclonal antibody. (Dilution 1:50).
MAP2K4 / MKK4 Antibody - IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-MAP2K4 mouse monoclonal antibody. (Dilution 1:50).
MAP2K4 / MKK4 Antibody - IHC of paraffin-embedded Carcinoma of liver tissue using anti-MAP2K4 mouse monoclonal antibody. (Dilution 1:50).
MAP2K4 / MKK4 Antibody - IHC of paraffin-embedded liver tissue using anti-MAP2K4 mouse monoclonal antibody. (Dilution 1:50).
MAP2K4 / MKK4 Antibody - Anti-MAP2K4 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAP2K4.
MAP2K4 / MKK4 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP2K4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP2K4.
MAP2K4 / MKK4 Antibody - Immunoprecipitation(IP) of MAP2K4 by using monoclonal anti-MAP2K4 antibodies (Negative control: IP without adding anti-MAP2K4 antibody.). For each experiment, 500ul of DDK tagged MAP2K4 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-MAP2K4 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
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Monoclonal Mouse anti‑Human MAP2K4 / MKK4 Antibody (clone OTI1D6, IHC, IF, WB) LS‑C114531

Monoclonal Mouse anti‑Human MAP2K4 / MKK4 Antibody (clone OTI1D6, IHC, IF, WB) LS‑C114531

Antibody:
MAP2K4 / MKK4 Mouse anti-Human Monoclonal (OTI1D6) Antibody
Application:
IHC, IHC-P, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$434
LS-C114531-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
MAP2K4 / MKK4 Mouse anti-Human Monoclonal (OTI1D6) Antibody
Application:
IHC, IHC-P, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
MKK4 antibody LS-C114531 is an unconjugated mouse monoclonal antibody to human MKK4 (MAP2K4). Validated for IF, IHC, IP and WB.
Target
Human MAP2K4 / MKK4
Synonyms
MAP2K4 | JNK activating kinase 1 | JNK-activated kinase 1 | JNK-activating kinase 1 | MAPK/ERK kinase 4 | MAPKK4 | JNKK1 | PRKMK4 | SAPKK1 | SEK1 | MEK4 | MKK4 | SAPK/ERK kinase 1 | SERK1 | JNKK | MAP kinase kinase 4 | MAPKK 4 | MEK 4 | SAPK kinase 1 | SAPKK-1 | SKK1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal
Clone
OTI1D6
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified. Also available Carrier-free.
Immunogen
Protein expressed in 293T cell transfected with human MAP2K4 expression vector.
Specificity
Human MKK4
Applications
  • IHC
  • IHC - Paraffin (1:150)
  • Immunofluorescence (1:100)
  • Western blot (1:2000)
  • Immunoprecipitation
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MAP2K4 / MKK4
P45985 NM_003010 NP_003001.1

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 4/16/2024