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Catalog Number Size Price
LS-C745380-100 100 µg (1 mg/ml) $485 
MAP2K2 / MKK2 / MEK2 Antibody - Immunohistochemistry with anti-MEK2 (N-Term) antibody showing positive staining in human glioblastoma tissue at 20x and 40x (B & C). Staining was performed on Leica Bond system using the standard protocol. Formalin fixed/paraffin embedded tissue sections were subjected to antigen retrieval and then incubated with rabbit anti-MEK2 (N-Term) antibody at 1:100 dilution for 60 minutes. Biotinylated Anti-rabbit secondary antibody was used at 1:200 dilution to detect primary antibody. The reaction was developed using streptavidin-HRP conjugated compact polymer system and visualized with chromogen substrate, 3?-diamino-benzidine substrate (DAB). The sections were then counterstained with hematoxylin to detect cell nuclei.
MAP2K2 / MKK2 / MEK2 Antibody - Western Blot of Anti-MEK2 N-term Antibody. Lane 1: MEK1 rec lysate. Lane 2: MEK2 rec lysate. Lane 3: Mouse Brain Whole cell lysate. Lane 4: HEK293T Whole cell lysate. Load: 10ug. Primary Antibody: Anti-MEK2 at 1µg/mL overnight at 4°C. Secondary Antibody: Goat Anti-Rabbit Peroxidase Conjugated Antibody at 1:40,000 for 30 min at RT. Blocking: BlockOut Universal Blocking blocking buffer MB-073. Predicted MW: 45kDa.
MAP2K2 / MKK2 / MEK2 Antibody - Immunohistochemistry with anti-MEK2 (N-Term) antibody showing positive staining in human glioblastoma tissue at 20x and 40x (B & C). Staining was performed on Leica Bond system using the standard protocol. Formalin fixed/paraffin embedded tissue sections were subjected to antigen retrieval and then incubated with rabbit anti-MEK2 (N-Term) antibody at 1:100 dilution for 60 minutes. Biotinylated Anti-rabbit secondary antibody was used at 1:200 dilution to detect primary antibody. The reaction was developed using streptavidin-HRP conjugated compact polymer system and visualized with chromogen substrate, 3?-diamino-benzidine substrate (DAB). The sections were then counterstained with hematoxylin to detect cell nuclei.
MAP2K2 / MKK2 / MEK2 Antibody - Western Blot of Anti-MEK2 N-term Antibody. Lane 1: MEK1 rec lysate. Lane 2: MEK2 rec lysate. Lane 3: Mouse Brain Whole cell lysate. Lane 4: HEK293T Whole cell lysate. Load: 10ug. Primary Antibody: Anti-MEK2 at 1µg/mL overnight at 4°C. Secondary Antibody: Goat Anti-Rabbit Peroxidase Conjugated Antibody at 1:40,000 for 30 min at RT. Blocking: BlockOut Universal Blocking blocking buffer MB-073. Predicted MW: 45kDa.
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Polyclonal Rabbit anti‑Human MAP2K2 / MKK2 / MEK2 Antibody (N‑Terminus, IHC, WB) LS‑C745380

Polyclonal Rabbit anti‑Human MAP2K2 / MKK2 / MEK2 Antibody (N‑Terminus, IHC, WB) LS‑C745380

Antibody:
MAP2K2 / MKK2 / MEK2 Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-C745380-100
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
MAP2K2 / MKK2 / MEK2 Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
MEK2 antibody LS-C745380 is an unconjugated rabbit polyclonal antibody to MEK2 (MAP2K2 / MKK2) (N-Terminus) from human. It is reactive with human, mouse and rat. Validated for ELISA, IHC and WB.
Target
Human MAP2K2 / MKK2 / MEK2
Synonyms
MAP2K2 | ERK activator kinase 2 | MAP kinase kinase 2 | MAPK/ERK kinase 2 | MAPKK 2 | MKK2 | MEK 2 | MEK2 | MAPKK2 | PRKMK2
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Predicted
Human, Mouse, Rat (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Anti-MEK2 Antibody was produced in rabbits by repeated immunizations with synthetic peptide corresponding to amino acid residues near the N-Terminus conjugated to KLH.
Epitope
N-Terminus
Specificity
Cross reactivity is expected to occur with human, mouse and rat based on sequence identity of the peptide immunogen. This antibody does not react with the MEK1 isoform.
Applications
  • IHC (1:100)
  • Western blot (1 µg/ml)
  • ELISA (1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MAP2K2 / MKK2 / MEK2
P36507 NM_030662 NP_109587.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/11/2021