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Catalog Number Size Price
LS-C745387-100 100 µg (1 mg/ml) $485 
MAP2K1 / MKK1 / MEK1 Antibody - Immunohistochemistry with anti-MEK1 (C-Term) antibody showing positive staining in human glioblastoma tissue at 20x and 40x (B & C). Staining was performed on Leica Bond system using the standard protocol. Formalin fixed/paraffin embedded tissue sections were subjected to antigen retrieval and then incubated with rabbit anti-MEK1 (C-Term) antibody at 1:100 dilution for 60 minutes. Biotinylated Anti-rabbit secondary antibody was used at 1:200 dilution to detect primary antibody. The reaction was developed using streptavidin-HRP conjugated compact polymer system and visualized with chromogen substrate, 3?-diamino-benzidine substrate (DAB). The sections were then counterstained with hematoxylin to detect cell nuclei.
MAP2K1 / MKK1 / MEK1 Antibody - Western Blot of Anti-MEK1 Antibody. Lane 1: Molecular Weight Marker. Lane 2: MEK1 recombinant lysate. Lane 3: MEK2 recombinant lysate. Load: 10µg. Primary Antibody: Anti-MEK1 (c-term) at 1µg/mL overnight at 4°C. Secondary Antibody: Goat anti-Rabbit peroxidase conjugated antibody at 1:40,000 for 30 minutes RT. Blocking: BlockOut Universal Blocking buffer MB-073. Predicted Size: 43.5kDa.
MAP2K1 / MKK1 / MEK1 Antibody - Western Blot of rabbit anti-MEK1 antibody. Marker: Opal Pre-stained ladder Lane 1: HEK293 lysate Lane 2: HeLa Lysate Lane 3: MCF-7 Lysate Lane 4: Jurkat Lysate Lane 5: A431 Lysate Lane 6: A549 Lysate Lane 7: LNCap Lysate Lane 8: MOLT-4 Lysate Lane 9: Ramos Lysate Lane 10: Raji Lsyate Lane 11: A-172 Lysate Lane 12: NIH/3T3 Lysate Load: 35 µg per lane. Primary antibody: MEK1 antibody at 1ug/mL overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted/Observed size: 43kDa for MEK1.
MAP2K1 / MKK1 / MEK1 Antibody - Immunohistochemistry with anti-MEK1 (C-Term) antibody showing positive staining in human glioblastoma tissue at 20x and 40x (B & C). Staining was performed on Leica Bond system using the standard protocol. Formalin fixed/paraffin embedded tissue sections were subjected to antigen retrieval and then incubated with rabbit anti-MEK1 (C-Term) antibody at 1:100 dilution for 60 minutes. Biotinylated Anti-rabbit secondary antibody was used at 1:200 dilution to detect primary antibody. The reaction was developed using streptavidin-HRP conjugated compact polymer system and visualized with chromogen substrate, 3?-diamino-benzidine substrate (DAB). The sections were then counterstained with hematoxylin to detect cell nuclei.
MAP2K1 / MKK1 / MEK1 Antibody - Western Blot of Anti-MEK1 Antibody. Lane 1: Molecular Weight Marker. Lane 2: MEK1 recombinant lysate. Lane 3: MEK2 recombinant lysate. Load: 10µg. Primary Antibody: Anti-MEK1 (c-term) at 1µg/mL overnight at 4°C. Secondary Antibody: Goat anti-Rabbit peroxidase conjugated antibody at 1:40,000 for 30 minutes RT. Blocking: BlockOut Universal Blocking buffer MB-073. Predicted Size: 43.5kDa.
MAP2K1 / MKK1 / MEK1 Antibody - Western Blot of rabbit anti-MEK1 antibody. Marker: Opal Pre-stained ladder Lane 1: HEK293 lysate Lane 2: HeLa Lysate Lane 3: MCF-7 Lysate Lane 4: Jurkat Lysate Lane 5: A431 Lysate Lane 6: A549 Lysate Lane 7: LNCap Lysate Lane 8: MOLT-4 Lysate Lane 9: Ramos Lysate Lane 10: Raji Lsyate Lane 11: A-172 Lysate Lane 12: NIH/3T3 Lysate Load: 35 µg per lane. Primary antibody: MEK1 antibody at 1ug/mL overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted/Observed size: 43kDa for MEK1.
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Polyclonal Rabbit anti‑Human MAP2K1 / MKK1 / MEK1 Antibody (C‑Terminus, IHC, WB) LS‑C745387

Polyclonal Rabbit anti‑Human MAP2K1 / MKK1 / MEK1 Antibody (C‑Terminus, IHC, WB) LS‑C745387

Antibody:
MAP2K1 / MKK1 / MEK1 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-C745387-100
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
MAP2K1 / MKK1 / MEK1 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
MEK1 antibody LS-C745387 is an unconjugated rabbit polyclonal antibody to MEK1 (MAP2K1 / MKK1) (C-Terminus) from human. It is reactive with human, mouse and rat. Validated for ELISA, IHC and WB.
Target
Human MAP2K1 / MKK1 / MEK1
Synonyms
MAP2K1 | ERK activator kinase 1 | MAPK/ERK kinase 1 | MAPKK 1 | MKK1 | PRKMK1 | MEK 1 | MAP kinase kinase 1 | MAPKK1 | MEK1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Predicted
Human, Mouse, Rat (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Anti-MEK1 Antibody was produced in rabbits by repeated immunizations with synthetic peptide corresponding to amino acid residues near the C-Terminus conjugated to KLH.
Epitope
C-Terminus
Specificity
Cross reactivity is expected to occur with human, mouse and rat based on sequence identity of the peptide immunogen. This antibody does not react with the MEK2 isoform.
Applications
  • IHC (1:100)
  • Western blot (1 µg/ml)
  • ELISA (1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MAP2K1 / MKK1 / MEK1
Q02750 NM_002755 NP_002746.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/17/2021