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Antibodies > MAP1LC3B / LC3B > LS-C168823
 
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Catalog Number Size Price
LS-C168823-400 400 µl $393 
MAP1LC3B / LC3B Antibody - SY5Y cells were pretreated with 5nM bafilomycin for 24hr and fixed in methanol (left panel) or 4% of paraformaldehyde (right panel). Treatment with antibody at dilution 1:100. Data courtesy of Jianhui Zhu, MD, PhD & Charleen T. Chu, MD, PhD, University of Pittsburgh School of Medicine.
MAP1LC3B / LC3B Antibody - Time course study of mouse leukemic monocyte macrophage cells treated with U18666A, a drug that causes cholesterol and lipid storage in cells, thereby blocking fusion between late endosomes and lysosomes. Cleaved-LC3 (APG8b) antibody detected punctuate staining indicative of autophagic vacuole or phagosome structures. Data courtesy of Dr. Barry Boland, Department of Pharmacology, Oxford University.
MAP1LC3B / LC3B Antibody - Fluorescent image of U251 cells stained with cleaved LC3B antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated cleaved LC3B primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
MAP1LC3B / LC3B Antibody - SY5Y cells were pretreated with 5nM bafilomycin for 24hr and fixed in methanol (left panel) or 4% of paraformaldehyde (right panel). Treatment with antibody at dilution 1:100. Data courtesy of Jianhui Zhu, MD, PhD & Charleen T. Chu, MD, PhD, University of Pittsburgh School of Medicine.
MAP1LC3B / LC3B Antibody - Time course study of mouse leukemic monocyte macrophage cells treated with U18666A, a drug that causes cholesterol and lipid storage in cells, thereby blocking fusion between late endosomes and lysosomes. Cleaved-LC3 (APG8b) antibody detected punctuate staining indicative of autophagic vacuole or phagosome structures. Data courtesy of Dr. Barry Boland, Department of Pharmacology, Oxford University.
MAP1LC3B / LC3B Antibody - Fluorescent image of U251 cells stained with cleaved LC3B antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated cleaved LC3B primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
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Polyclonal Rabbit anti‑Human MAP1LC3B / LC3B Antibody (aa89‑122, IHC, IF) LS‑C168823

Polyclonal Rabbit anti‑Human MAP1LC3B / LC3B Antibody (aa89‑122, IHC, IF) LS‑C168823

Submit Review LSBio Guarantee
Antibody:
MAP1LC3B / LC3B Rabbit anti-Human Polyclonal (aa89-122) Antibody
Application:
IHC, ICC, IF
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C168823-400
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
MAP1LC3B / LC3B Rabbit anti-Human Polyclonal (aa89-122) Antibody
Application:
IHC, ICC, IF
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
LC3B antibody LS-C168823 is an unconjugated rabbit polyclonal antibody to LC3B (MAP1LC3B) (aa89-122) from human. It is reactive with human and mouse. Validated for ICC, IF and IHC.
Target
Human MAP1LC3B / LC3B
Synonyms
MAP1LC3B | ATG8F | LC3B | MAP1A/1BLC3 | MAP1A/MAP1B LC3 B | MAP1A/MAP1B light chain 3 B | MAP1ALC3 | MAP1LC3B-a
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Predicted
Cow (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Epitope
aa89-122
Specificity
This Cleaved LC3B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-122 amino acids from human Cleaved LC3B.
Applications
  • IHC
  • ICC (1:10 - 1:50)
  • Immunofluorescence (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MAP1LC3B / LC3B
Q9GZQ8 NM_022818 NP_073729.1

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MAP1LC3B / LC3B Antibody (aa25‑121, Biotin) LS‑C83721
Species: Human, Mouse, Rat, Dog, Fish, Zebrafish
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation
MAP1LC3B / LC3B Antibody - Anti-MAP1LC3B / LC3B antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. This image was taken for the unconjugated form of this product. Other forms have not been tested.
IHC‑plus™ MAP1LC3B / LC3B Antibody (N‑Terminus) LS‑B9650
Species: Human, Mouse, Rat, Bovine, Dog, Pig, Zebrafish, Primate
Applications: IHC, IHC - Paraffin, IHC - Frozen, Immunofluorescence, Flow Cytometry
MAP1LC3B / LC3B Antibody - Anti-MAP1LC3B / LC3B antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
IHC‑plus™ MAP1LC3B / LC3B Antibody (Thr6) LS‑B9807
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot
MAP1LC3B / LC3B Antibody - Anti-MAP1LC3B / LC3B antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
IHC‑plus™ MAP1LC3B / LC3B Antibody (aa100‑125) LS‑B9581
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
Product
MAP1LC3B / LC3B Antibody (aa1‑72) LS‑C308873
Species: Human
Applications: ELISA
MAP1LC3B / LC3B Antibody - MAP1LC3A antibody (5 ug/ml) was used in IHC to stain formalin-fixed, paraffin-embedded human brain, cortex, followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. This image was taken for the unconjugated form of this product. Other forms have not been tested.
MAP1LC3B / LC3B Antibody (aa1‑100, Biotin) LS‑C83716
Species: Human, Rat, Zebrafish
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation
See all MAP1LC3B / LC3B Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
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