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Catalog Number Size Price
LS-C775629-100 100 µg (1 mg/ml) $535 
Malondialdehyde Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Malondialdehyde Monoclonal Antibody, Clone 6H6. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Malondialdehyde Monoclonal Antibody  at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 µM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alexa Fluor 633 F-Actin stain. (E,F) Malondialdehyde Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
Malondialdehyde Antibody - Western Blot analysis of Malondialdehyde-BSA Conjugate showing detection of 67 kDa Malondialdehyde protein using Mouse Anti-Malondialdehyde Monoclonal Antibody, Clone 6H6. Lane 1: Molecular Weight Ladder (MW). Lane 2: Malondialdehyde-BSA (0.5 µg). Lane 3: Malondialdehyde-BSA (2.0 µg). Lane 4: BSA (0.5 µg). Lane 5: BSA (2.0 µg). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Malondialdehyde Monoclonal Antibody  at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
Malondialdehyde Antibody - Flow Cytometry analysis using Mouse Anti-Malondialdehyde Monoclonal Antibody, Clone 6H6. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-Malondialdehyde Monoclonal Antibody  at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 µM H2O2 for 24 hours.
Malondialdehyde Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Malondialdehyde Monoclonal Antibody, Clone 6H6. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Malondialdehyde Monoclonal Antibody  at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 µM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alexa Fluor 633 F-Actin stain. (E,F) Malondialdehyde Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
Malondialdehyde Antibody - Western Blot analysis of Malondialdehyde-BSA Conjugate showing detection of 67 kDa Malondialdehyde protein using Mouse Anti-Malondialdehyde Monoclonal Antibody, Clone 6H6. Lane 1: Molecular Weight Ladder (MW). Lane 2: Malondialdehyde-BSA (0.5 µg). Lane 3: Malondialdehyde-BSA (2.0 µg). Lane 4: BSA (0.5 µg). Lane 5: BSA (2.0 µg). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Malondialdehyde Monoclonal Antibody  at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
Malondialdehyde Antibody - Flow Cytometry analysis using Mouse Anti-Malondialdehyde Monoclonal Antibody, Clone 6H6. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-Malondialdehyde Monoclonal Antibody  at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 µM H2O2 for 24 hours.
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Monoclonal Mouse anti‑All species Malondialdehyde Antibody (clone 6H6, Atto 390, IF, WB) LS‑C775629

Monoclonal Mouse anti‑All species Malondialdehyde Antibody (clone 6H6, Atto 390, IF, WB) LS‑C775629

Antibody:
Malondialdehyde Mouse Monoclonal (Atto 390) (6H6) Antibody
Application:
IF, WB, Flo, ELISA
Reactivity:
All species
Format:
Atto 390, Unmodified
Other formats:
Price
Catalog Number
$535
LS-C775629-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
Malondialdehyde Mouse Monoclonal (Atto 390) (6H6) Antibody
Application:
IF, WB, Flo, ELISA
Reactivity:
All species
Format:
Atto 390, Unmodified
Other formats:

Specifications

Description
Malondialdehyde antibody LS-C775629 is an Atto 390-conjugated mouse monoclonal antibody to all species Malondialdehyde. Validated for ELISA, Flow, IF and WB.
Target
All species Malondialdehyde
Host
Mouse
Reactivity
All species
Clonality
IgG1 Monoclonal
Clone
6H6
Conjugations
Atto 390. Also available conjugated with AP, APC, Atto 488, Atto 594, Biotin, FITC, PerCP, RPE.
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Synthetic Malondialdehyde modified Keyhole Limpet Hemocyanin (KLH).
Specificity
Specific for Malondialdehyde conjugated proteins. Does not detect free Malondialdehyde. Does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, or Methylglyoxal modified proteins.
Applications
  • Immunofluorescence (1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:50)
  • ELISA (1:1000)
  • Applications tested for the base form of this product only
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
Storage
Store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Requested From: United States
Date Requested: 4/19/2024