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Catalog Number Size Price
LS-C331254-20 20 µl $275 
LS-C331254-50 50 µl $311 
LS-C331254-100 100 µl $389 
LS-C331254-200 200 µl $503 
MAGED1 / NRAGE Antibody - Immunohistochemistry of paraffin-embedded human liver cancer using MAGED1 antibodyat dilution of 1:100 (40x lens).
MAGED1 / NRAGE Antibody - Immunofluorescence analysis of U2OS cells.
MAGED1 / NRAGE Antibody - Western blot analysis of extracts of various cell lines, using MAGED1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
MAGED1 / NRAGE Antibody - Western blot analysis of A549 cell lysate.
MAGED1 / NRAGE Antibody - Immunohistochemistry of paraffin-embedded human liver cancer using MAGED1 antibodyat dilution of 1:100 (40x lens).
MAGED1 / NRAGE Antibody - Immunofluorescence analysis of U2OS cells.
MAGED1 / NRAGE Antibody - Western blot analysis of extracts of various cell lines, using MAGED1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
MAGED1 / NRAGE Antibody - Western blot analysis of A549 cell lysate.
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Polyclonal Rabbit anti‑Human MAGED1 / NRAGE Antibody (IHC, IF, WB) LS‑C331254

Polyclonal Rabbit anti‑Human MAGED1 / NRAGE Antibody (IHC, IF, WB) LS‑C331254

Antibody:
MAGED1 / NRAGE Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C331254-20
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
MAGED1 / NRAGE Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
NRAGE antibody LS-C331254 is an unconjugated rabbit polyclonal antibody to NRAGE (MAGED1) from human. It is reactive with human, mouse and rat. Validated for IF, IHC and WB.
Target
Human MAGED1 / NRAGE
Synonyms
MAGED1 | DLXIN-1 | MAGE-D1 antigen | Melanoma antigen family D, 1 | NRAGE | MAGE tumor antigen CCF | Melanoma-associated antigen D1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 635-834 of human MAGED1 (NP_001005333.1). EAVLWEALRKMGLRPGVRHPLLGDLRKLLTYEFVKQKYLDYRRVPNSNPPEYEFLWGLRSYHETSKMKVLRFIAEVQKRDPRDWTAQFMEAADEALDALDAAAAEAEARAEARTRMGIGDEAVSGPWSWDDIEFELLTWDEEGDFGDPWSRIPFTFWARYHQNARSRFPQTFAGPIIGPGGTASANFAANFGAIGFFWVE
Specificity
Human MAGED1 / NRAGE
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:50 - 1:200)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The predicted MW is 86kDa/91kDa, while the observed MW by Western blot was 86kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MAGED1 / NRAGE

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/15/2024