Human Macrophage + Histiocytoma Marker

Mouse

Human (tested or 100% immunogen sequence identity)

Mouse, Rat, Pig

IgG1,k Monoclonal

D11

Unconjugated

Protein G purified

Unmodified. Also available Azide-free, BSA-free, Ready-to-Use.

Membrane preparation from human hepatocytes.

Human Macrophage + Histiocytoma Marker

• IHC
• IHC - Paraffin (0.5 - 1 µg/ml)

In Western blotting, it detects an antigen of 125kDa in human liver and 135kDa in tumors of histiocytic origin. Comparative study of this mAb and a standard CD68 mAb showed that their antigens are different. Its antigen in all macrophage types studied is located on the plasma membrane and within cytoplasmic structures including lysosomes. This mAb shows a restricted reactivity to cells of the monocyte/macrophage system. It specifically reacts with blood monocytes and stains resident macrophages in a wide variety of human tissues. This mAb does not stain antigen-presenting cells, e.g., Langerhans cells. Reportedly, its reactivity is restricted to histiocytes and macrophages. Optimal dilution of the Macrophage / Histiocytoma Marker antibody should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.