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Catalog Number Size Price
LS-C744954-25 25 µl $275 
LYZ / Lysozyme Antibody - Western Blot of rabbit anti-Lysozyme (Hen Egg White) Antibody. Lane 1: Lysozyme (Hen Egg White). Load: 50 ng per lane. Primary antibody: Rabbit anti-Lysozyme (Hen Egg White) Antibody at 1:1,000 overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 4.9 kDa, 4.9 kDa for Lysozyme (Hen Egg White).
LYZ / Lysozyme Antibody - Western Blot of rabbit anti-Lysozyme antibody. (Left Blot-grey) Samples of ~1 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with Biotin Conjugated Rabbit anti Lysozyme antibody. Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Load: ~1 ug per lane. Primary antibody: Biotin Conjugated Rabbit anti Lysozyme antibody at 1:5000 for overnight at 4°C. Secondary antibody: Dylight 488 conjugated Streptavidin at 1:1,000 with Block: MB-070 overnight at 4°C. (Right Blot-red) Blot shown on right shows detection of 0.5 ug reduced (R) and nonreduced (NR) Lysozyme using p/n 200-401-072 lot 26517 (1:1000 in MB-070) with Dylight 549 conjugated secondary antibody. Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Predicted/Observed size: 4.9kDa, 5 kDa for Lysozyme. Other band(s): none.
LYZ / Lysozyme Antibody - Western Blot of rabbit anti-Lysozyme (Hen Egg White) Antibody. Lane 1: Lysozyme (Hen Egg White). Load: 50 ng per lane. Primary antibody: Rabbit anti-Lysozyme (Hen Egg White) Antibody at 1:1,000 overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 4.9 kDa, 4.9 kDa for Lysozyme (Hen Egg White).
LYZ / Lysozyme Antibody - Western Blot of rabbit anti-Lysozyme antibody. (Left Blot-grey) Samples of ~1 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with Biotin Conjugated Rabbit anti Lysozyme antibody. Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Load: ~1 ug per lane. Primary antibody: Biotin Conjugated Rabbit anti Lysozyme antibody at 1:5000 for overnight at 4°C. Secondary antibody: Dylight 488 conjugated Streptavidin at 1:1,000 with Block: MB-070 overnight at 4°C. (Right Blot-red) Blot shown on right shows detection of 0.5 ug reduced (R) and nonreduced (NR) Lysozyme using p/n 200-401-072 lot 26517 (1:1000 in MB-070) with Dylight 549 conjugated secondary antibody. Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Predicted/Observed size: 4.9kDa, 5 kDa for Lysozyme. Other band(s): none.
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Polyclonal Rabbit anti‑Chicken LYZ / Lysozyme Antibody (IHC, WB) LS‑C744954

Polyclonal Rabbit anti‑Chicken LYZ / Lysozyme Antibody (IHC, WB) LS‑C744954

Antibody:
LYZ / Lysozyme Rabbit anti-Chicken Polyclonal Antibody
Application:
IHC, WB, IP, ELISA
Reactivity:
Chicken
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C744954-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
LYZ / Lysozyme Rabbit anti-Chicken Polyclonal Antibody
Application:
IHC, WB, IP, ELISA
Reactivity:
Chicken
Format:
Unconjugated, Unmodified

Specifications

Description
Lysozyme antibody LS-C744954 is an unconjugated rabbit polyclonal antibody to chicken Lysozyme (LYZ). Validated for ELISA, IHC, IP and WB.
Target
Chicken LYZ / Lysozyme
Synonyms
LYZ | 1,4-beta-N-acetylmuramidase C | LZM | Lysozyme | Lysozyme (renal amyloidosis) | Renal amyloidosis | Lysozyme C | Gal d 4 | P00698
Host
Rabbit
Reactivity
Chicken (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Unmodified
Immunogen
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length protein corresponding to amino acids 1-129 of Hen Egg White Lysozyme.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Lysozyme (Hen Egg White). A BLAST analysis was used to suggest that this antibody would react with all forms of lysozyme from chicken, including precursor as well as A and B chains. Chains designated as M, L, F, C, Y and D also show 100% sequence homology. Lysozyme from quail and pheasant are also reported to be 100% identical with HEW lysozyme. Cross reactivity against lysozyme from other sources may occur but has not been specifically determined.
Applications
  • IHC (1:500 - 1:2000)
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation (1:100)
  • ELISA (1:25000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About LYZ / Lysozyme
P61626 NM_000239 NP_000230.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/14/2021