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LYZ / Lysozyme Antibody (aa1‑129) LS‑C153796

Catalog Number Size Price
LS-C153796-100 100 µg (1 mg/ml) $425 
Western blot of Rabbit anti-Lysozyme antibody. (Left Blot-grey) Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Load: 0.5 ug per lane. Primary antibody: Biotin Conjugated Rabbit anti Lysozyme antibody at 1:5000 for overnight at 4C. Secondary antibody: Dylight 488 conjugated Streptavidin at 1:5000 with Atto 425 conjugated goat anti rabbit secondary antibody at 1:10000 for 1.5 hrs at RT. (Right Blot-red) Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Secondary antibody: Dylight 488 conjugated Streptavidin at 1:5000 with Dylight 549 conjugated secondary antibody at 1:10000 for 1.5 hrs at RT. Block: MB-070 overnight at 4C. Predicted/Observed size: 4.9kDa, 5kDa for Lysozyme. Other band(s): none.

LYZ / Lysozyme Antibody (aa1‑129) LS‑C153796

Antibody:
Rabbit Polyclonal to Chicken LYZ / Lysozyme
Application:
IHC, WB, IP, ELISA
Reactivity:
Chicken
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C153796-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Chicken LYZ / Lysozyme
Application:
IHC, WB, IP, ELISA
Reactivity:
Chicken
Format:
Unconjugated, Unmodified

Specifications

Description
Lysozyme antibody LS-C153796 is an unconjugated rabbit polyclonal antibody to chicken Lysozyme (LYZ ). Validated for ELISA, IHC, IP and WB.
Target
Chicken LYZ / Lysozyme
Host
Rabbit
Reactivity
Chicken (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Ion exchange chromatography
Modifications
Unmodified
Applications
  • IHC (1:500 - 1:2000)
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation (1:100)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length protein corresponding to amino acids 1-129 of Hen Egg White Lysozyme.
Epitope
aa1-129
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Lysozyme (Hen Egg White). A BLAST analysis was used to suggest that this antibody would react with all forms of lysozyme from chicken, including precursor as well as A and B chains. Chains designated as M, L, F, C, Y and D also show 100% sequence homology. Lysozyme from quail and pheasant are also reported to be 100% identical with HEW lysozyme. Cross reactivity against lysozyme from other sources may occur but has not been specifically determined.
Usage
This purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~14 kD in size corresponding to lysozyme by western blotting in the appropriate cell lysate or extract.
Presentation
Sterile Solution 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About LYZ / Lysozyme
P61626 NM_000239 NP_000230.1

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Western Blot of Rabbit anti-Lysozyme antibody. (Left Blot-grey) Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. (Right Blot-red) Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Load: ~1 ug per lane. Primary antibody: Biotin Conjugated Rabbit anti Lysozyme antibody at 1:5000 for overnight at 4 degrees C. Secondary antibody: Dylight 488 conjugated Streptavidin at 1:5,000 with Dylight 549 conjugated secondary antibody at 1:10,000 for 1.5 hrs at RT. Block: MB-070 overnight at 4 degrees C. Predicted/Observed size: 4.9kDa, 5kDa for Lysozyme. Other band(s): none. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Requested From: United States
Date Requested: 8/21/2019
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