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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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LSS Antibody (N‑Terminus) LS‑C80464
LSS antibody LS-C80464 is an unconjugated rabbit polyclonal antibody to LSS from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C80464-50
50 µg
Unavailable

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Product Description

LSS antibody LS-C80464 is an unconjugated rabbit polyclonal antibody to LSS from human, mouse, rat and other species. Validated for WB.
About LSS
LSS catalyzes the conversion of (S)-2,3 oxidosqualene to lanosterol. The encoded protein is a member of the terpene cyclase/mutase family and catalyzes the first step in the biosynthesis of cholesterol, steroid hormones, and vitamin D. Alternative splicing results in multiple transcript variants encoding different isoforms. P48449 NM_002340 NP_002331.3

LSS Antibody, Lanosterol synthase Antibody, HOSC Antibody, OSC Antibody


Specifications

Target
Human LSS
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Guinea pig, Hamster, Rabbit, Zebrafish (tested or 100% immunogen sequence identity)
Predicted
Horse, Xenopus (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Failed Applications
  • IHC - Paraffin
Immunogen
LSS antibody was raised against synthetic peptide from N-Terminus of human LSS (P48449, NP_002331). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Galago, Mouse, Rat, Hamster, Bovine, Bat, Rabbit, Guinea pig, Lizard, Stickleback, Zebrafish (100%); Elephant, Horse, Opossum, Xenopus (92%).
Blocking Peptide
LS-E15393 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C80464. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
N-Terminus
Specificity
Human LSS
Usage
ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
50 µl Distilled Water.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images

Western blot

Fetal liver lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal liver lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Fetal liver lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal liver lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Fetal liver lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal liver lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Fetal liver lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal liver lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 7/16/2018

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