Human LRRC32

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2a,k Monoclonal

ANC8C9

Unconjugated. Also available conjugated with FITC, RPE, Biotin.

Protein A purified

Unmodified. Also available Preservative Free.

Recombinant human GARP.

Antibody ANC8C9 binds to recombinant GARP in EIA and to cell surface GARP expressed on a subset of FOXP3 positive cells.

• Flow Cytometry
• ELISA

(FACS analysis) Ficoll prepared human PBMC were stimulated in culture overnight with immobilized anti-human CD3 antibody, anti-human CD28 mAb, and human IL-2. Cells were harvested and 5 x 105 cells per tube were washed and pre incubated ~5 minutes with 20 ul human IgG at 250 ug/ml (to block non specific binding ) after which they were incubated 45 minutes on ice with 80 ul of anti-CD4/APC conjugate and anti-human GARP/Biotin at a concentration of 5 ug/ml. Cells were washed twice and incubated with 50 ul of 2o reagent Streptavidin/PE, after which they were washed three times, fixed and analyzed by FACS, gating on CD4 positive lymphoid cells. A net 4.7 % sub population of gated cells stained positive with a mean shift of 1.59 log10 fluorescent units when compared to a Mouse IgG2a/Biotin isotype control. This binding was blocked when cells were pre incubated with 50 ug/ml unlabeled anti-human GARP mAb.

50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl, 0.5 mg/ml Gentamicin

Store at 4°C, avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q14392 NCBI: NM_005512 NP_005503.1