LRP6 antibody LS-B5 is an unconjugated goat polyclonal antibody to LRP6 from human, rat, dog and other species. Validated for IHC and Peptide-ELISA. Tested on 20 paraffin-embedded human tissues.
Human, Monkey, Rat, Dog, Hamster, Horse, Pig, Rabbit
(tested or 100% immunogen sequence identity)
Mouse, Bat, Bovine (at least 90% immunogen sequence identity)
- IHC - Paraffin (5 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
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LRP6 antibody was raised against synthetic peptide CMTSVATAKGYTSDL from an internal region of human LRP6 (NP_002327.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Rat, Hamster, Panda, Dog, Horse, Rabbit, Pig (100%); Mouse, Elephant, Bovine, Bat (93%).
- Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B5. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Human LRP6. This antibody is expected to recognise an epitope corresponding to aa 1546-1560 of human LRP6 protein.
Immunohistochemistry: LS-B5 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B5 was determined to be 5 ug/ml.
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.