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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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LMNA / Lamin A+C Antibody LS‑C204529
Lamin A+C antibody LS-C204529 is an unconjugated chicken polyclonal antibody to Lamin A+C (LMNA) from human, mouse, rat and other species. Validated for ICC, IF and WB.
Catalog
Size
Price
LS-C204529-100
100 µl
$285
Description
Lamin A+C antibody LS-C204529 is an unconjugated chicken polyclonal antibody to Lamin A+C (LMNA) from human, mouse, rat and other species. Validated for ICC, IF and WB.
Target
Human LMNA / Lamin A+C
Host
Chicken
Reactivity
Human, Mouse, Rat, Bovine, Pig (tested or 100% immunogen sequence identity)
Clonality
IgY Polyclonal
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Applications
  • ICC (1:1000)
  • Immunofluorescence (1:1000)
  • Western blot (1:2000)
Immunogen
Recombinant full length purified human Lamin A from E. coli.
Specificity
This antibody is known to react with Lamin A/C from human, cow, pig, mouse, rat and other mammals. Since Lamin A/C is highly conserved, it is likely that the antibody is effective on other species also.
Usage
The antibody solution can be used at dilutions of at least 1:1000 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:2000.
Presentation
10 mM Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About LMNA / Lamin A+C
P02545 NM_005572 NP_005563.1

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Images

Immunocytochemistry

HeLa cells staining with LMNA / Lamin A/C antibody (red), and counterstained with 6E2 monoclonal antibody to Lysosomal Associated Membrane Protein 1 (Lamp1, green) and DNA (blue). The LMNA / Lamin A/C antibody antibody reveals strong nuclear lamina staining, while MCA-6E2 antibody reveals strong cytoplasmic punctate staining of lysosomes and early endosomes. Since both DNA (blue) and Lamin A/C (red) are associated with the nuclear compartment, this region appears crimson in this image.
HeLa cells staining with LMNA / Lamin A/C antibody (red), and counterstained with 6E2 monoclonal antibody to Lysosomal Associated Membrane Protein 1 (Lamp1, green) and DNA (blue). The LMNA / Lamin A/C antibody antibody reveals strong nuclear lamina staining, while MCA-6E2 antibody reveals strong cytoplasmic punctate staining of lysosomes and early endosomes. Since both DNA (blue) and Lamin A/C (red) are associated with the nuclear compartment, this region appears crimson in this image.

Western blot

Stripe blot of crude HeLa cell extract stained with LMNA / Lamin A/C antibody. Note two strong clean bands at 74kDa and 65kDa, corresponding to Lamins A and C.
Stripe blot of crude HeLa cell extract stained with LMNA / Lamin A/C antibody. Note two strong clean bands at 74kDa and 65kDa, corresponding to Lamins A and C.

Immunocytochemistry

HeLa cells staining with LMNA / Lamin A/C antibody (red), and counterstained with 6E2 monoclonal antibody to Lysosomal Associated Membrane Protein 1 (Lamp1, green) and DNA (blue). The LMNA / Lamin A/C antibody antibody reveals strong nuclear lamina staining, while MCA-6E2 antibody reveals strong cytoplasmic punctate staining of lysosomes and early endosomes. Since both DNA (blue) and Lamin A/C (red) are associated with the nuclear compartment, this region appears crimson in this image.
HeLa cells staining with LMNA / Lamin A/C antibody (red), and counterstained with 6E2 monoclonal antibody to Lysosomal Associated Membrane Protein 1 (Lamp1, green) and DNA (blue). The LMNA / Lamin A/C antibody antibody reveals strong nuclear lamina staining, while MCA-6E2 antibody reveals strong cytoplasmic punctate staining of lysosomes and early endosomes. Since both DNA (blue) and Lamin A/C (red) are associated with the nuclear compartment, this region appears crimson in this image.

Western blot

Stripe blot of crude HeLa cell extract stained with LMNA / Lamin A/C antibody. Note two strong clean bands at 74kDa and 65kDa, corresponding to Lamins A and C.
Stripe blot of crude HeLa cell extract stained with LMNA / Lamin A/C antibody. Note two strong clean bands at 74kDa and 65kDa, corresponding to Lamins A and C.

Immunocytochemistry

HeLa cells staining with LMNA / Lamin A/C antibody (red), and counterstained with 6E2 monoclonal antibody to Lysosomal Associated Membrane Protein 1 (Lamp1, green) and DNA (blue). The LMNA / Lamin A/C antibody antibody reveals strong nuclear lamina staining, while MCA-6E2 antibody reveals strong cytoplasmic punctate staining of lysosomes and early endosomes. Since both DNA (blue) and Lamin A/C (red) are associated with the nuclear compartment, this region appears crimson in this image.
HeLa cells staining with LMNA / Lamin A/C antibody (red), and counterstained with 6E2 monoclonal antibody to Lysosomal Associated Membrane Protein 1 (Lamp1, green) and DNA (blue). The LMNA / Lamin A/C antibody antibody reveals strong nuclear lamina staining, while MCA-6E2 antibody reveals strong cytoplasmic punctate staining of lysosomes and early endosomes. Since both DNA (blue) and Lamin A/C (red) are associated with the nuclear compartment, this region appears crimson in this image.

Western blot

Stripe blot of crude HeLa cell extract stained with LMNA / Lamin A/C antibody. Note two strong clean bands at 74kDa and 65kDa, corresponding to Lamins A and C.
Stripe blot of crude HeLa cell extract stained with LMNA / Lamin A/C antibody. Note two strong clean bands at 74kDa and 65kDa, corresponding to Lamins A and C.

Immunocytochemistry

HeLa cells staining with LMNA / Lamin A/C antibody (red), and counterstained with 6E2 monoclonal antibody to Lysosomal Associated Membrane Protein 1 (Lamp1, green) and DNA (blue). The LMNA / Lamin A/C antibody antibody reveals strong nuclear lamina staining, while MCA-6E2 antibody reveals strong cytoplasmic punctate staining of lysosomes and early endosomes. Since both DNA (blue) and Lamin A/C (red) are associated with the nuclear compartment, this region appears crimson in this image.
HeLa cells staining with LMNA / Lamin A/C antibody (red), and counterstained with 6E2 monoclonal antibody to Lysosomal Associated Membrane Protein 1 (Lamp1, green) and DNA (blue). The LMNA / Lamin A/C antibody antibody reveals strong nuclear lamina staining, while MCA-6E2 antibody reveals strong cytoplasmic punctate staining of lysosomes and early endosomes. Since both DNA (blue) and Lamin A/C (red) are associated with the nuclear compartment, this region appears crimson in this image.

Western blot

Stripe blot of crude HeLa cell extract stained with LMNA / Lamin A/C antibody. Note two strong clean bands at 74kDa and 65kDa, corresponding to Lamins A and C.
Stripe blot of crude HeLa cell extract stained with LMNA / Lamin A/C antibody. Note two strong clean bands at 74kDa and 65kDa, corresponding to Lamins A and C.

Requested From: United States
Date Requested: 11/18/2018
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