Human LMNA / Lamin A+C
Human, Monkey, Mouse, Rat, Dog, Hamster, Horse, Pig, Rabbit
(tested or 100% immunogen sequence identity)
Bovine (at least 90% immunogen sequence identity)
- IHC - Paraffin (1:100)
- Western blot (1:500 - 1:1000)
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including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
A synthetic peptide corresponding to amino acids 249-265 C-RAQHEDQVEQYKKELEK of Human Lamin A/C. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Horse, Rabbit, Pig (100%); Bovine, Opossum (94%); Bat, Platypus (88%).
Because of immunogen's 100% homology with Isoforms 1, 4, 5, 6, ADelta10 and C of Prelamin-A/C, this is expected to detect multiple bands in WB.
Immunohistochemistry: LS-B119 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B119 was determined to be 1:100.
PBS, 0.05% Sodium Azide, 0.05% BSA
Short term: 4°C; Long term: -70°C.
For research use only.