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Catalog Number Size Price
LS-C745202-25 25 µl (1 mg/ml) $275 
LINE-1 Antibody - Western blot using the IgY fraction of anti-L1/ORF2 antibody shows detection of induced bacterially expressed human ORF2 (left lane). No specific band staining is seen in the uninduced lane (right lane). The lower molecular weight bands represent non-specific staining. The band at ~70 kDa corresponds to a human L1/ORF2 EN domain fusion protein (arrowhead).

Polyclonal Chicken anti‑Human LINE‑1 Antibody (WB) LS‑C745202

Polyclonal Chicken anti‑Human LINE‑1 Antibody (WB) LS‑C745202

Antibody:
LINE-1 Chicken anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human, Chimpanzee
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C745202-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
LINE-1 Chicken anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human, Chimpanzee
Format:
Unconjugated, Unmodified

Specifications

Description
LINE-1 antibody LS-C745202 is an unconjugated chicken polyclonal antibody to LINE-1 from human. It is reactive with human and chimpanzee. Validated for ELISA and WB.
Target
Human LINE-1
Host
Chicken
Reactivity
Human, Chimpanzee (tested or 100% immunogen sequence identity)
Predicted
Chimpanzee (at least 90% immunogen sequence identity)
Clonality
IgY Polyclonal
Conjugations
Unconjugated
Purification
Selective precipitation and salt fractionation followed by daialysis.
Modifications
Unmodified
Immunogen
This IgY fraction antibody was prepared from eggs of chickens laid after repeated immunizations with two synthetic peptides conjugated to keyhole limpet hemocyanin (KLH).  The peptides correspond to regions within the endonuclease domain of L1/ORF2 protein.
Specificity
A BLAST analysis was used to suggest cross reactivity with L1/ORF2 proteins from chimpanzee sources based on 100% homology with the immunizing sequences, and from macaque, fruit fly, cattle, dog, opossum, and rat sources based on 69 - 88% homology with the immunizing sequences.
Applications
  • Western blot (1:2000 - 1:10000)
  • ELISA (1:10000)
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/11/2021