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| Catalog Number | Size | Price |
|---|---|---|
| LS-C154072-100 | 100 µg (1 mg/ml) | $567 |
Polyclonal Chicken anti‑Human LINE‑1 Antibody (WB) LS‑C154072
Polyclonal Chicken anti‑Human LINE‑1 Antibody (WB) LS‑C154072
Antibody:
LINE-1 Chicken anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human, Chimpanzee
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
LINE-1 Chicken anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human, Chimpanzee
Format:
Unconjugated, Unmodified
Specifications
Description
LINE-1 antibody LS-C154072 is an unconjugated chicken polyclonal antibody to LINE-1 from human. It is reactive with human and chimpanzee. Validated for ELISA and WB.
Host
Chicken
Reactivity
Human, Chimpanzee
(tested or 100% immunogen sequence identity)
Predicted
Chimpanzee (at least 90% immunogen sequence identity)
Clonality
IgY
Polyclonal
Conjugations
Unconjugated
Purification
Selective precipitation and salt fractionation followed by daialysis.
Modifications
Unmodified
Immunogen
This IgY fraction antibody was prepared from eggs of chickens laid after repeated immunizations with two synthetic peptides conjugated to keyhole limpet hemocyanin (KLH). The peptides correspond to regions within the endonuclease domain of L1/ORF2 protein.
Specificity
A BLAST analysis was used to suggest cross reactivity with L1/ORF2 proteins from chimpanzee sources based on 100% homology with the immunizing sequences, and from macaque, fruit fly, cattle, dog, opossum, and rat sources based on 69 - 88% homology with the immunizing sequences.
Applications
- Western blot (1:2000 - 1:10000)
- ELISA (1:10000)
Usage
This IgY fraction antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 149 kD in size corresponding to L1 protein by western blotting in the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
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Featured Products
Reactivity:
Human
Range:
0.156-10 ng/ml
Reactivity:
Monkey
Range:
0.156-10 ng/ml
Reactivity:
Monkey
Range:
31.25-2000 pg/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
Date Requested: 4/19/2024
