Human LIMK1 / LIMK
LIMK1 | LIM domain kinase 1 | Lim kinase 1 | LIMK-1 | LIM kinase | LIMK
Human, Chimpanzee, Orangutan
(tested or 100% immunogen sequence identity)
Chimpanzee, Orangutan, Mouse, Rat (at least 90% immunogen sequence identity)
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminal region near aa 620-647 of human LIM kinase protein.
A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee and orangutan based on 100% homology for the immunogen sequence. Expect cross reactivity with LIM kinase from mouse and rat sources as only a single amino acid residue change is found within the immunogen sequence (94% positive by BLAST). Cross reactivity with LIM kinase homologues from other sources has not been determined.
- Western blot (1:500 - 1:2000)
- ELISA (1:8000 - 1:36000)
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 73 kD in size corresponding to LIM kinase by western blotting in the appropriate cell lysate or extract.
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
For research use only. Intended for use by laboratory professionals.
This antibody carries the LSBio 100% Guarantee.