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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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LGMN / Legumain Antibody IHC‑plus™ LS‑B15611
Note: This antibody replaces LS-C497277
Legumain antibody LS-B15611 is an unconjugated rabbit polyclonal antibody to Legumain (LGMN) from human, mouse and rat. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B15611-50
50 µl
$425

Popular LGMN / Legumain Products

LGMN / Legumain antibody Western Blot of Fetal Brain.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Rabbit, Sheep
Applications: Western blot
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE) This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Anti-LGMN / Legumain antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Mouse
Applications: ICC, Western blot

Product Description

Legumain antibody LS-B15611 is an unconjugated rabbit polyclonal antibody to Legumain (LGMN) from human, mouse and rat. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
About LGMN / Legumain
LGMN / Legumain is a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. Q99538 NM_005606 NP_005597.3

LGMN Antibody, AEP Antibody, LGMN1 Antibody, Protease, cysteine 1 Antibody, Asparaginyl endopeptidase Antibody, Cysteine protease 1 Antibody, Legumain Antibody, PRSC1 Antibody


Specifications

Target
Human LGMN / Legumain
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:200)
  • Western blot (1:1000 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
LGMN / Legumain antibody was raised against recombinant fusion protein containing a sequence corresponding to amino acids 18-323 of human LGMN (NP_001008530.1). VPIDDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQIIHRNGIPDEQIVVMMYDDIAYSEDNPTPGIVINRPNGTDVYQGVPKDYTGEDVTPQNFLAVLRGDAEAVKGIGSGKVLKSGPQDHVFIYFTDHGSTGILVFPNEDLHVKDLNETIHYMYKHKMYRKMVFYIEACESGSMMNHLPDNINVYATTAANPRESSYACYYDEKRSTYLGDWYSVNWMEDSDVEDLTKETLHKQYHLVKSHTNTSHVMQYGNKTISTMKVMQFQGMKRKASSPVPLPPVTHLDLTPSPDVPLTIMKRKLMNTN
Usage
The predicted MW is 42kDa/49kDa, while the observed MW by Western blot was 54kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.

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Images

Immunohistochemistry - Paraffin

Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry - Paraffin

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western blot

Western blot analysis of extracts of various cell lines, using LGMN antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 1s.
Western blot analysis of extracts of various cell lines, using LGMN antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 1s.

Immunohistochemistry - Paraffin

Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry - Paraffin

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western blot

Western blot analysis of extracts of various cell lines, using LGMN antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 1s.
Western blot analysis of extracts of various cell lines, using LGMN antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 1s.

Immunohistochemistry - Paraffin

Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry - Paraffin

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western blot

Western blot analysis of extracts of various cell lines, using LGMN antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 1s.
Western blot analysis of extracts of various cell lines, using LGMN antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 1s.

Immunohistochemistry - Paraffin

Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry - Paraffin

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western blot

Western blot analysis of extracts of various cell lines, using LGMN antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 1s.
Western blot analysis of extracts of various cell lines, using LGMN antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 1s.

Requested From: United States
Date Requested: 9/22/2018

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