Human Leptin

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

Unconjugated

Protein A purified

Unmodified

E.coli derived recombinant Human Leptin.

Human Leptin

• IHC
• IHC - Paraffin (10 µg/ml)
• Western blot (0.2 - 0.4 µg/ml)
• ELISA (12 - 16 µg/ml)

Immunohistochemistry: LS-B8192 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 10 ug/ml is recommended. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 2-4 ug/ml of this antibody will detect recombinant human Leptin when used with biotinylated antigen affinity purified anti-human Leptin as the detection antibody at a concentration of approximately 0.5-1 ug/ml. Western Blot: To detect hLeptin by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLeptin is 2-4 ng/lane, under non-reducing conditions.

Lyophilized from PBS

Reconstitute with sterile water

Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P41159 NCBI: NM_000230 NP_000221.1