Leptin antibody LS-B1459 is an unconjugated rabbit polyclonal antibody to Leptin from human and mouse. Validated for ELISA, IHC and WB. Cited in 1 publication.
(tested or 100% immunogen sequence identity)
- IHC - Paraffin (5 µg/ml)
- Western blot
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Leptin antibody was raised against recombinant mouse LEP / Leptin.
Recombinant mouse leptin 16000 MW produced in E. coli.
Immunohistochemistry: LS-B1459 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1459 was determined to be 5 ug/ml.
0.01% Sodium Azide
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
For research use only.