Leptin antibody was raised against recombinant mouse LEP / Leptin.
Recombinant mouse leptin 16000 MW produced in E. coli.
Immunohistochemistry: LS-B1459 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1459 was determined to be 5 ug/ml.
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
This antibody carries the LSBio 100% Guarantee.