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Catalog Number Size Price
LS-C744804-25 25 µl (1 mg/ml) $275 
LDHA / LDH1 Antibody - Immunofluorescence Microscopy of Biotin conjugated Anti-Lactate Dehydrogenase Antibody. Tissue: HeLa cells. Fixation: fixed for 5 min in 1:1 MeTOH:Acetone, blocked with MB-071 (preservative free) for 15 min. Antigen retrieval: not required. Primary antibody: Lactate Dehydrogenase antibody at 1:200 for 1 h at RT. Secondary antibody: DyLight 488 conjugated Streptavidin antibody at 1:10,000 for 30 min at RT. Staining: Lactate Dehydrogenase as green fluorescent signal.
LDHA / LDH1 Antibody - Western Blot of Goat anti-Lactate Dehydrogenase Antibody Peroxidase Conjugated. Lane 1: Lactate Dehydrogenase (Rabbit Muscle). Load: 50 ng per lane. Primary antibody: Goat anti-Lactate Dehydrogenase Antibody Peroxidase Conjugated at 1:1,000 overnight at 4°C. Secondary antibody: n/a. Block: MB-070 for 30 min at RT. Predicted/Observed size: 36.5 kDa, 36.5 kDa for Lactate Dehydrogenase.
LDHA / LDH1 Antibody - Western Blot of Goat Anti-Lactate Dehydrogenase antibody. Lane 1-4: HeLa cell extracts cytoplasmic fraction (CF). Lane 5-8: HeLa cell extracts nuclear fraction (NF). Load: 30 µg per lane. Primary antibody: LDH antibody at 1:400 for overnight at 4°C. Secondary antibody: secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO/TBST overnight at 4°C. Predicted/Observed size: 36.6 kDa, 36 kDa for LDH. Other band(s): None.
LDHA / LDH1 Antibody - Immunofluorescence Microscopy of Biotin conjugated Anti-Lactate Dehydrogenase Antibody. Tissue: HeLa cells. Fixation: fixed for 5 min in 1:1 MeTOH:Acetone, blocked with MB-071 (preservative free) for 15 min. Antigen retrieval: not required. Primary antibody: Lactate Dehydrogenase antibody at 1:200 for 1 h at RT. Secondary antibody: DyLight 488 conjugated Streptavidin antibody at 1:10,000 for 30 min at RT. Staining: Lactate Dehydrogenase as green fluorescent signal.
LDHA / LDH1 Antibody - Western Blot of Goat anti-Lactate Dehydrogenase Antibody Peroxidase Conjugated. Lane 1: Lactate Dehydrogenase (Rabbit Muscle). Load: 50 ng per lane. Primary antibody: Goat anti-Lactate Dehydrogenase Antibody Peroxidase Conjugated at 1:1,000 overnight at 4°C. Secondary antibody: n/a. Block: MB-070 for 30 min at RT. Predicted/Observed size: 36.5 kDa, 36.5 kDa for Lactate Dehydrogenase.
LDHA / LDH1 Antibody - Western Blot of Goat Anti-Lactate Dehydrogenase antibody. Lane 1-4: HeLa cell extracts cytoplasmic fraction (CF). Lane 5-8: HeLa cell extracts nuclear fraction (NF). Load: 30 µg per lane. Primary antibody: LDH antibody at 1:400 for overnight at 4°C. Secondary antibody: secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO/TBST overnight at 4°C. Predicted/Observed size: 36.6 kDa, 36 kDa for LDH. Other band(s): None.
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Polyclonal Goat anti‑Rabbit LDHA / LDH1 Antibody (Liquid, HRP, IHC, IF, WB) LS‑C744804

Polyclonal Goat anti‑Rabbit LDHA / LDH1 Antibody (Liquid, HRP, IHC, IF, WB) LS‑C744804

Antibody:
LDHA / LDH1 Goat anti-Rabbit Polyclonal (Liquid) (HRP) Antibody
Application:
IHC, IF, WB, IP, ELISA
Reactivity:
Rabbit
Format:
Horseradish Peroxidase, Liquid
Price
Catalog Number
$275
LS-C744804-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
LDHA / LDH1 Goat anti-Rabbit Polyclonal (Liquid) (HRP) Antibody
Application:
IHC, IF, WB, IP, ELISA
Reactivity:
Rabbit
Format:
Horseradish Peroxidase, Liquid

Specifications

Description
LDH1 antibody LS-C744804 is an HRP-conjugated goat polyclonal antibody to rabbit LDH1 (LDHA). Validated for ELISA, IF, IHC, IP and WB.
Target
Rabbit LDHA / LDH1
Synonyms
LDHA | LDH muscle subunit | LDH1 | Lactate dehydrogenase M | LDH-M | GSD11 | Lactate dehydrogenase A | LDH-A | LDHM | PIG19 | Proliferation-inducing gene 19
Host
Goat
Reactivity
Rabbit (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Horseradish Peroxidase
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Liquid
Immunogen
Anti-Lactate Dehydrogenase Antibody was produced by repeated immunizations with rabbit muscle Lactate Dehydrogenase full length protein.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Lactate Dehydrogenase [Rabbit Muscle]. Cross reactivity against Lactate Dehydrogenase from other tissues and species may occur but have not been specifically determined.
Applications
  • IHC (1:500 - 1:2500)
  • Immunofluorescence
  • Western blot (1:1000 - 1:6000)
  • Immunoprecipitation (1:100)
  • ELISA (1:3000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About LDHA / LDH1
P00338 NM_005566 NP_005557.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/14/2021