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Antibodies > LDH / Lactate Dehydrogenase > LS-C59234
 
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Catalog Number Size Price
LS-C59234-2 2 ml (82 mg/ml) $1,225 
LDH / Lactate Dehydrogenase Antibody - Lactate Dehydrogenase Antibody-Immunofluorescence. Biotin conjugated anti-lactate dehydrogenase antibody was used to stain HeLa cells by immunofluorescence. HeLa cells were plated in 12 well plates, fixed for 5 min in 1:1 Methanol: Acetone, blocked with MB-071 (preservative free) for 15 min and stained 1 hr with antibody 1:200 in blocking buffer. Plate was washed 3X in PBS, and primary biotin conjugate was detected by DyLight 488 conjugated Streptavidin (S000-41 lot 21097) 1:10000 for 30 min. Well was washed 3X in PBS.
LDH / Lactate Dehydrogenase Antibody - Anti-Lactate Dehydrogenase Antibody - Western Blot. Western blot analysis shows Anti-Lactate Dehydrogenase antibody detects LDH in HeLa cell extracts. Reactivity with LDH is observed in the cytoplasmic fraction (CF) and little to no reactivity in the nuclear fraction (NF). ~30 ug was loaded per lane onto a 10% gel for SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a single band of ~36 kD corresponding to the expected molecular weight for the protein. The blot was incubated with a 1:1000 dilution of the antibody in 5% milk in TBST at room temperature followed by detection using standard procedures. Personal communication Kuldeep Patel.
LDH / Lactate Dehydrogenase Antibody - Lactate Dehydrogenase Antibody-Immunofluorescence. Biotin conjugated anti-lactate dehydrogenase antibody was used to stain HeLa cells by immunofluorescence. HeLa cells were plated in 12 well plates, fixed for 5 min in 1:1 Methanol: Acetone, blocked with MB-071 (preservative free) for 15 min and stained 1 hr with antibody 1:200 in blocking buffer. Plate was washed 3X in PBS, and primary biotin conjugate was detected by DyLight 488 conjugated Streptavidin (S000-41 lot 21097) 1:10000 for 30 min. Well was washed 3X in PBS.
LDH / Lactate Dehydrogenase Antibody - Anti-Lactate Dehydrogenase Antibody - Western Blot. Western blot analysis shows Anti-Lactate Dehydrogenase antibody detects LDH in HeLa cell extracts. Reactivity with LDH is observed in the cytoplasmic fraction (CF) and little to no reactivity in the nuclear fraction (NF). ~30 ug was loaded per lane onto a 10% gel for SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a single band of ~36 kD corresponding to the expected molecular weight for the protein. The blot was incubated with a 1:1000 dilution of the antibody in 5% milk in TBST at room temperature followed by detection using standard procedures. Personal communication Kuldeep Patel.
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Polyclonal Goat anti‑Rabbit LDH / Lactate Dehydrogenase Antibody (IF, WB) LS‑C59234

Polyclonal Goat anti‑Rabbit LDH / Lactate Dehydrogenase Antibody (IF, WB) LS‑C59234

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Note: This antibody replaces LS-C25046
Antibody:
LDH / Lactate Dehydrogenase Goat anti-Rabbit Polyclonal Antibody
Application:
IF, WB, IP, ELISA
Reactivity:
Rabbit
Format:
Unconjugated, Unmodified
Price
Catalog Number
$1,225
LS-C59234-2
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
LDH / Lactate Dehydrogenase Goat anti-Rabbit Polyclonal Antibody
Application:
IF, WB, IP, ELISA
Reactivity:
Rabbit
Format:
Unconjugated, Unmodified

Specifications

Description
Lactate Dehydrogenase antibody LS-C59234 is an unconjugated goat polyclonal antibody to rabbit Lactate Dehydrogenase (LDH). Validated for ELISA, IF, IP and WB.
Target
Rabbit LDH / Lactate Dehydrogenase
Host
Goat
Reactivity
Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Delipidated and defibrinated
Modifications
Unmodified
Immunogen
This antibody was prepared from whole goat serum produced by repeated immunizations with a full length lactate dehydrogenase protein isolated from rabbit muscle.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Lactate Dehydrogenase [Rabbit Muscle]. BLAST analysis was used to determine that cross reactivity is suggested for both muscle and heart isoforms (LDH-A and LDH-B) from most mammalian species.
Applications
  • Immunofluorescence
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation (1:100)
  • ELISA (1:4000 - 1:20000)
Usage
This antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 36 kD in size corresponding to LDH western blotting in the appropriate cell lysate or extract.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 2 mL of deionized water.
Storage
Store at 4°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/24/2024
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