Mouse LDB1 / CLIM2

Rabbit

Mouse, Human, Rat (tested or 100% immunogen sequence identity)

Human, Chimpanzee, Rat, Dog, Chicken, Frog (at least 90% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region of mouse LDB1 protein.

C-Terminus

A BLAST analysis was used to suggest reactivity with this protein from mouse, human, chimpanzee, dog, frog, chicken and rat based on 100% homology for the immunogen sequence. Cross reactivity with LDB1 homologues from other sources has not been determined.

• Western blot (1:500 - 1:3000)
• ELISA (1:425000)
• Chromatin Immunoprecipitation

This affinity purified antibody has been tested for use in ELISA, western blot and CHIP. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 43 kD in size corresponding to LDB1 by western blotting in the appropriate cell lysate or extract. This antibody has been used in a ChIP assay using murine erythroleukemia (MEL) cells. The test sequence was the upstream enhancer of the GATA-1 gene; a putative LDB1 binding region as suggested by Orkin et al. Anti-LDB1 was used successfully in ChIP assays to precipitate a roughly 4-fold enrichment at the GATA1-HS1 enhancer element in DMSO-induced murine erythroleukemia cells. We suggest using Use 20 ug for 108 cells for ChIP.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q86U70 NCBI: NM_003893 NP_003884.1