NGAL antibody LS-C16216 is an unconjugated mouse monoclonal antibody to human NGAL (LCN2 / Lipocalin 2). Validated for ELISA, IHC and WB.
Human LCN2 / Lipocalin 2 / NGAL
(tested or 100% immunogen sequence identity)
Protein A purified
- IHC - 4% paraformaldehyde
- Western blot (1:500)
- ELISA (1:16000)
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Native neutrophil gelatinase associated lipocalin isolated from neutrophils (1). Screened in a sandwich ELISA.
Recognizes human NGAL. Epitope specificity differs from that of clone 6F155 and 6F156. Strong reaction is seen when tested in sandwich ELISA in combination with a polyclonal NGAL capture antibody and when tested on recombinant NGAL coated directly onto the well. Reacts with NGAL in immunochemical staining of paraformaldehyde-fixed sections containing neutrophils. In Western blotting of SDS-PAGE gels, reacts with NGAL in both reduced and unreduced forms. No cross-reaction with NGAL from other species has been detected.
Suitable for use in ELISA, Western Blot and Immunohistochemistry. ELISA: 1:16000. Western Blot: 1:500.
Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
For research use only.
About LCN2 / Lipocalin 2 / NGAL