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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-B3144-50 50 µg (1 mg/ml) $425 
LAMP2 / CD107b Antibody - Anti-LAMP2 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

IHCPlus™ LAMP2 / CD107b Antibody Monoclonal Flo,IF,IHC,IP,WB LS‑B3144

IHCPlus™ LAMP2 / CD107b Antibody Monoclonal Flo,IF,IHC,IP,WB LS‑B3144

Note: This antibody replaces LS-C71900
Antibody:
Mouse Monoclonal to Dog LAMP2 / CD107b
Application:
IHC, IHC-P, IF, WB, IP, Flo
Reactivity:
Dog, Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$425 
LS-B3144-50
50 µg
1 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Dog LAMP2 / CD107b
Application:
IHC, IHC-P, IF, WB, IP, Flo
Reactivity:
Dog, Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
CD107b antibody LS-B3144 is an unconjugated mouse monoclonal antibody to CD107b (LAMP2 ) from human and dog. Validated for Flow, IF, IHC, IP and WB. Cited in 2 publications.
Target
Dog LAMP2 / CD107b
Host
Mouse
Reactivity
Dog, Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Unconjugated. Also available conjugated with AP, Biotin, FITC, HRP, PE.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Immunofluorescence
  • Western blot
  • Immunoprecipitation
  • Flow Cytometry
  • Applications tested for the base form of this product only
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
MDCK (Madin-Darby Canine Kidney) cells.
Specificity
Recognizes canine CD107b, otherwise known as LAMP-2, a 95kD heavily-glycosylated type I integral lysosomal glycoprotein, shown to share high N-terminal amino acid sequence homology with human, mouse and rat CD107b. Species sequence homology: human, mink. Does not react with mouse or rat.
Usage
Immunohistochemistry: LS-B3144 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3144 was determined to be 10 ug/ml. Flow Cytometry: 10 ul labels 1x10^6 cells in 100ul. Membrane permeabilization is required for this application.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About LAMP2 / CD107b
P13473 NM_013995 NP_054701.1

Validation

LAMP2 / CD107b Antibody - Anti-LAMP2 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-LAMP2 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-LAMP2 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Publications (2)

A missense change in the ATG4D gene links aberrant autophagy to a neurodegenerative vacuolar storage disease. Kyöstilä K, Syrjä P, Jagannathan V, Chandrasekar G, Jokinen TS, Seppälä EH, Becker D, Drögemüller M, Dietschi E, Drögemüller C, Lang J, Steffen F, Rohdin C, Jäderlund KH, Lappalainen AK, Hahn K, Wohlsein P, Baumgärtner W, Henke D, Oevermann A, Kere J, Lohi H, Leeb T. PLoS genetics. 2015 11:e1005169. (IHC; Canine)

Databases for technical aspects of immunohistochemistry. Furukawa S, Nagaike M, Ozaki K. Journal of toxicologic pathology. 2017 30:79-107. (IHC; Canine)

Customer Reviews (0)


Requested From: United States
Date Requested: 12/12/2019