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Catalog Number Size Price
LS-C783540-100 100 µg (0.5 mg/ml) $285 
LAMP1 / CD107a Antibody - Formalin-fixed paraffin-embedded human tonsil treated with a citrate buffer pH6 and heat for antigen retrieval was stained with purified CD107a clone H4A3, conjugated and detected with a Cy5 conjugated CODEX™ oligonucleotide duplex (green). Data generated at Akoya Biosciences, Inc. using the CODEX™ technology.
LAMP1 / CD107a Antibody - ICC staining of purified anti-LAMP-1 antibody (clone H4A3) on Hela cells. The cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then stained with 5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-Mouse IgG for one hour at room temperature. Cells were counterstained with Phalloidin™ Green 488 and DAPI to visualize actin filaments and nuclei, respectively. The images were captured with a 60X objective. Scale bar: 50 µm.
LAMP1 / CD107a Antibody - Western blot of purified anti-LAMP-1 antibody (clone H4A3). Lane 1: Molecular weight marker; Lane 2: 20 µg of Hela cell lysate; Lane 3: 20 µg of NIH3T3 cell lysate; Lane 4: 20 µg of human brain lysate; Lane 5: 20 µg of mouse brain lysate; Lane 6: 10 ng of human recombinant LAMP-1. The blot was incubated with 1 µg/ml of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG. Anti-ß-actin antibody was used as the loading control. Enhanced chemiluminescence was used as the detection system.
LAMP1 / CD107a Antibody - Thrombin-activated human peripheral blood platelets were stained with purified CD107a (clone H4A3) (red histogram) or purified mouse IgG1, kappa (green histogram), followed by anti-mouse IgG FITC.
LAMP1 / CD107a Antibody - Formalin-fixed paraffin-embedded human tonsil treated with a citrate buffer pH6 and heat for antigen retrieval was stained with purified CD107a clone H4A3, conjugated and detected with a Cy5 conjugated CODEX™ oligonucleotide duplex (green). Data generated at Akoya Biosciences, Inc. using the CODEX™ technology.
LAMP1 / CD107a Antibody - ICC staining of purified anti-LAMP-1 antibody (clone H4A3) on Hela cells. The cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then stained with 5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-Mouse IgG for one hour at room temperature. Cells were counterstained with Phalloidin™ Green 488 and DAPI to visualize actin filaments and nuclei, respectively. The images were captured with a 60X objective. Scale bar: 50 µm.
LAMP1 / CD107a Antibody - Western blot of purified anti-LAMP-1 antibody (clone H4A3). Lane 1: Molecular weight marker; Lane 2: 20 µg of Hela cell lysate; Lane 3: 20 µg of NIH3T3 cell lysate; Lane 4: 20 µg of human brain lysate; Lane 5: 20 µg of mouse brain lysate; Lane 6: 10 ng of human recombinant LAMP-1. The blot was incubated with 1 µg/ml of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG. Anti-ß-actin antibody was used as the loading control. Enhanced chemiluminescence was used as the detection system.
LAMP1 / CD107a Antibody - Thrombin-activated human peripheral blood platelets were stained with purified CD107a (clone H4A3) (red histogram) or purified mouse IgG1, kappa (green histogram), followed by anti-mouse IgG FITC.
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Monoclonal Mouse anti‑Human LAMP1 / CD107a Antibody (clone H4A3, IHC, WB) LS‑C783540

Monoclonal Mouse anti‑Human LAMP1 / CD107a Antibody (clone H4A3, IHC, WB) LS‑C783540

Antibody:
LAMP1 / CD107a Mouse anti-Human Monoclonal (H4A3) Antibody
Application:
IHC-P, ICC, WB, IP, Flo
Reactivity:
Human, Chimpanzee, Baboon, African green monkey, Cynomolgus monkey, Pig-tailed macaque, Rhesus monkey
Format:
Unconjugated, Unmodified
Price
Catalog Number
$285
LS-C783540-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
LAMP1 / CD107a Mouse anti-Human Monoclonal (H4A3) Antibody
Application:
IHC-P, ICC, WB, IP, Flo
Reactivity:
Human, Chimpanzee, Baboon, African green monkey, Cynomolgus monkey, Pig-tailed macaque, Rhesus monkey
Format:
Unconjugated, Unmodified

Specifications

Description
CD107a antibody LS-C783540 is an unconjugated mouse monoclonal antibody to CD107a (LAMP1) from human. It is reactive with human, african green monkey, baboon and other species. Validated for Flow, ICC, IHC, IP and WB.
Target
Human LAMP1 / CD107a
Synonyms
LAMP1 | CD107a | CD107a antigen | LAMP-1 | LGP120 | LAMPA
Host
Mouse
Reactivity
Human, Chimpanzee, Baboon, African green monkey, Cynomolgus monkey, Pig-tailed macaque, Rhesus monkey (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
H4A3
Conjugations
Unconjugated
Purification
Purified by affinity chromatography.
Modifications
Unmodified
Immunogen
Human adult adherent peripheral blood cells
Applications
  • IHC - Paraffin (5 - 10 µg/ml)
  • ICC
  • Western blot (1 - 5 µg/ml)
  • Immunoprecipitation
  • Flow Cytometry (2 µg/10E6 cells)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 2.0 µg per million cells in 100 µl volume. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For immunocytochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10 µg per ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application. Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining, immunofluorescence, and immunoprecipitation.This antibody is specific to human LAMP-1. Positive control: Hela cells; LAMP-1 molecular weight appears to be at ~110 kDa on the gel due to high glycosylation.
Presentation
PBS pH 7.2, 0.09% sodium azide.
Storage
Store at 2°C to 8°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About LAMP1 / CD107a
P11279 NM_005561 NP_005552.3

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Requested From: United States
Date Requested: 4/18/2024