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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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LAMC2 / Laminin Gamma 2 Antibody LS‑C674986
Laminin Gamma 2 antibody LS-C674986 is an unconjugated rabbit polyclonal antibody to human Laminin Gamma 2 (LAMC2). Validated for IHC and WB.
Catalog
Size
Price
LS-C674986-50
50 µg
$285
LS-C674986-100
100 µg
$385

Popular LAMC2 / Laminin Gamma 2 Products

IHC of paraffin-embedded BT483 xenograft using Laminin gamma 2 antibody at 1:250 dilution.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
LAMC2 / Laminin Gamma 2 antibody. IHC(P): Human Lung Cancer Tissue.
Species: Human, Monkey, Mouse, Rat, Bovine, Horse
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human
Applications: IHC, ICC, Western blot, Immunoprecipitation, ELISA
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Western blot analysis of extracts of rat lung cells.
Species: Human, Mouse, Rat
Applications: IHC, Western blot

Product Description

Laminin Gamma 2 antibody LS-C674986 is an unconjugated rabbit polyclonal antibody to human Laminin Gamma 2 (LAMC2). Validated for IHC and WB.

Specifications

Target
Human LAMC2 / Laminin Gamma 2
Synonyms
LAMC2, B2T, BM600, EBR2, CSF 140 kDa subunit, EBR2A, Epiligrin subunit gamma, LAMNB2, LAMB2T, Laminin B2t chain, Laminin gamma-2 chain, Nicein subunit gamma, Kalinin-105kDa, Ladsin 140 kDa subunit, BM600-100kDa, Kalinin subunit gamma, Laminin subunit gamma-2, Laminin, gamma 2, Laminin-5 subunit gamma, Nicein-100kDa
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with HRP, FITC, Biotin.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC - Paraffin
  • Western blot
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Recombinant Human Laminin subunit gamma-2 protein (417-588AA)
Presentation
0.01 M PBS, pH 7.4, 0.03% ProClin™ 300, 50% Glycerol
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Restrictions
For research use only.
About LAMC2 / Laminin Gamma 2
Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Q13753 NM_005562 NP_005553.2


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Images

Immunohistochemistry - Paraffin

Immunohistochemistry image at a dilution of 1:200 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunohistochemistry image at a dilution of 1:200 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunohistochemistry - Paraffin

Immunohistochemistry image at a dilution of 1:200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunohistochemistry image at a dilution of 1:200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Western blot

Positive Western Blot detected in Hela whole cell lysate. All lanes: LAMC2 antibody at 5 µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 131, 122 KDa. Observed band size: 140 KDa
Positive Western Blot detected in Hela whole cell lysate. All lanes: LAMC2 antibody at 5 µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 131, 122 KDa. Observed band size: 140 KDa

Immunohistochemistry - Paraffin

Immunohistochemistry image at a dilution of 1:200 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunohistochemistry image at a dilution of 1:200 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunohistochemistry - Paraffin

Immunohistochemistry image at a dilution of 1:200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunohistochemistry image at a dilution of 1:200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Western blot

Positive Western Blot detected in Hela whole cell lysate. All lanes: LAMC2 antibody at 5 µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 131, 122 KDa. Observed band size: 140 KDa
Positive Western Blot detected in Hela whole cell lysate. All lanes: LAMC2 antibody at 5 µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 131, 122 KDa. Observed band size: 140 KDa

Immunohistochemistry - Paraffin

Immunohistochemistry image at a dilution of 1:200 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunohistochemistry image at a dilution of 1:200 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunohistochemistry - Paraffin

Immunohistochemistry image at a dilution of 1:200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunohistochemistry image at a dilution of 1:200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Western blot

Positive Western Blot detected in Hela whole cell lysate. All lanes: LAMC2 antibody at 5 µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 131, 122 KDa. Observed band size: 140 KDa
Positive Western Blot detected in Hela whole cell lysate. All lanes: LAMC2 antibody at 5 µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 131, 122 KDa. Observed band size: 140 KDa

Immunohistochemistry - Paraffin

Immunohistochemistry image at a dilution of 1:200 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunohistochemistry image at a dilution of 1:200 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunohistochemistry - Paraffin

Immunohistochemistry image at a dilution of 1:200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunohistochemistry image at a dilution of 1:200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Western blot

Positive Western Blot detected in Hela whole cell lysate. All lanes: LAMC2 antibody at 5 µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 131, 122 KDa. Observed band size: 140 KDa
Positive Western Blot detected in Hela whole cell lysate. All lanes: LAMC2 antibody at 5 µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 131, 122 KDa. Observed band size: 140 KDa

Requested From: United States
Date Requested: 11/14/2018

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