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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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LAMB1 / Laminin Beta 1 Antibody (clone LT3) LS‑C25125 Best Seller
Laminin Beta 1 antibody LS-C25125 is an unconjugated rat monoclonal antibody to Laminin Beta 1 (LAMB1) from human, mouse, hamster and other species. Validated for IF, IHC, IP and WB.
Antibody
Catalog
Size
Price
LS-C25125-500
500 µl (0.2 mg/ml)
Unavailable

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Product Description

Laminin Beta 1 antibody LS-C25125 is an unconjugated rat monoclonal antibody to Laminin Beta 1 (LAMB1) from human, mouse, hamster and other species. Validated for IF, IHC, IP and WB.
About LAMB1 / Laminin Beta 1
Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. P07942 NM_002291 NP_002282.2

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Specifications

Target
Mouse LAMB1 / Laminin Beta 1
Host
Rat
Reactivity
Mouse, Human, Hamster, Pig (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [LT3]
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Frozen
  • Immunofluorescence
  • Western blot (1 - 2 µg/ml)
  • Immunoprecipitation
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Failed Applications
  • IHC - Paraffin
Immunogen
Murine EHS laminin preparation. Cellular Localization: Basement membrane.
Specificity
Recognizes the laminin B chain at ~210kD. Stains most human and mouse basement membranes. Does not stain glomerular and arterial membranes. Species cross-reactivity: Human, porcine, hamster and mouse.
Usage
Suitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Immunoprecipitation (Native and denatured): 2 ug antibody/mg protein lysate using Protein G. Western Blot: 1-2 ug/ml for 2 hrs at RT. Immunohistochemistry: 2 ug/ml for 30 min at RT (Acetone-fixed frozen and alcohol-fixed, paraffin-embedded). Positive control: LS174T cells. Normal colon or colon carcinoma.
Presentation
PBS, pH 7.4, 0.2% BSA, 0.1% sodium azide.
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 7/18/2018

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