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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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LAMA4 / Laminin Alpha 4 Antibody (aa25‑270, clone 5G8) LS‑C340651
Laminin Alpha 4 antibody LS-C340651 is an unconjugated mouse monoclonal antibody to human Laminin Alpha 4 (LAMA4). Validated for IHC and WB.
Catalog
Size
Price
LS-C340651-100
100 µl (1 mg/ml)
Unavailable

Popular LAMA4 / Laminin Alpha 4 Products

Immunohistochemistry analysis of paraffin-embedded human heart tissue, using LAMA4 Antibody. The picture on the right is blocked with the synthesized peptide.
Species: Human, Mouse
Applications: IHC, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Western blot of recombinant LAMA4 / Laminin Alpha 4.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse
Applications: IHC, Western blot
Immunohistochemical analysis of Laminin alpha 4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay

Product Description

Laminin Alpha 4 antibody LS-C340651 is an unconjugated mouse monoclonal antibody to human Laminin Alpha 4 (LAMA4). Validated for IHC and WB.
About LAMA4 / Laminin Alpha 4
Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. NM_002290 NP_002281.3

LAMA4 Antibody, Laminin alpha 4 chain Antibody, Laminin-8 subunit alpha Antibody, LAMA4*-1 Antibody, Laminin subunit alpha-4 Antibody, Laminin, alpha 4 Antibody, Laminin-14 subunit alpha Antibody, Laminin-9 subunit alpha Antibody


Specifications

Target
Human LAMA4 / Laminin Alpha 4
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [5G8]
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:150)
  • Western blot (1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Human recombinant protein fragment corresponding to amino acids 25-270 of human LAMA4(NP_001098676) produced in E. coli.
Epitope
aa25-270
Specificity
Human LAMA4 / Laminin Alpha 4
Presentation
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images

Immunohistochemistry

IHC of paraffin-embedded Human pancreas tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
IHC of paraffin-embedded Human pancreas tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

Immunohistochemistry

IHC of paraffin-embedded Human lymph node tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
IHC of paraffin-embedded Human lymph node tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human prostate tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
IHC of paraffin-embedded Carcinoma of Human prostate tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

Immunohistochemistry

IHC of paraffin-embedded Human prostate tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
IHC of paraffin-embedded Human prostate tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human liver tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
IHC of paraffin-embedded Carcinoma of Human liver tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

Immunohistochemistry

IHC of paraffin-embedded Human Kidney tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
IHC of paraffin-embedded Human Kidney tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LAMA4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LAMA4.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LAMA4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LAMA4.

Immunohistochemistry

IHC of paraffin-embedded Human pancreas tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
IHC of paraffin-embedded Human pancreas tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

Immunohistochemistry

IHC of paraffin-embedded Human lymph node tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
IHC of paraffin-embedded Human lymph node tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human prostate tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
IHC of paraffin-embedded Carcinoma of Human prostate tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

Immunohistochemistry

IHC of paraffin-embedded Human prostate tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
IHC of paraffin-embedded Human prostate tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human liver tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
IHC of paraffin-embedded Carcinoma of Human liver tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

Immunohistochemistry

IHC of paraffin-embedded Human Kidney tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
IHC of paraffin-embedded Human Kidney tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LAMA4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LAMA4.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LAMA4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LAMA4.

Immunohistochemistry

IHC of paraffin-embedded Human pancreas tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
IHC of paraffin-embedded Human pancreas tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

Immunohistochemistry

IHC of paraffin-embedded Human lymph node tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
IHC of paraffin-embedded Human lymph node tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human prostate tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
IHC of paraffin-embedded Carcinoma of Human prostate tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

Immunohistochemistry

IHC of paraffin-embedded Human prostate tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
IHC of paraffin-embedded Human prostate tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human liver tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
IHC of paraffin-embedded Carcinoma of Human liver tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

Immunohistochemistry

IHC of paraffin-embedded Human Kidney tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
IHC of paraffin-embedded Human Kidney tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LAMA4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LAMA4.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LAMA4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LAMA4.

Immunohistochemistry

IHC of paraffin-embedded Human pancreas tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
IHC of paraffin-embedded Human pancreas tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

Immunohistochemistry

IHC of paraffin-embedded Human lymph node tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
IHC of paraffin-embedded Human lymph node tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human prostate tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
IHC of paraffin-embedded Carcinoma of Human prostate tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

Immunohistochemistry

IHC of paraffin-embedded Human prostate tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
IHC of paraffin-embedded Human prostate tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human liver tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
IHC of paraffin-embedded Carcinoma of Human liver tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

Immunohistochemistry

IHC of paraffin-embedded Human Kidney tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
IHC of paraffin-embedded Human Kidney tissue using anti-LAMA4 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LAMA4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LAMA4.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LAMA4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LAMA4.

Requested From: 
Date Requested: 7/17/2018

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