LAG3 antibody LS-B2237 is an FITC-conjugated mouse monoclonal antibody to human LAG3. Validated for Flow and IHC. Tested on 20 paraffin-embedded human tissues. Cited in 4 publications.
(tested or 100% immunogen sequence identity)
- IHC - Paraffin (10 µg/ml)
- Flow Cytometry (1:100)
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LAG3 antibody was raised against synthetic peptide corresponding to 30 aa (GPPAAAPGHPLAPGPHPAAPSSWGPRPRRY) from the first N-terminal D1 domain of human LAG-3 (lymphocyte activation gene-3). Percent identity by BLAST analysis: Human (100%); Gorilla (93%); Gibbon (90%); Monkey (83%).
Recognizes the 30 aa extra-loop of the first N-terminal D1 domain of human LAG-3.
Immunohistochemistry: LS-B2237 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2237 was determined to be 10 ug/ml. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
PBS containing 50mM Tris-HCl, 1% BSA, 0.02% sodium azide.
Store at 4°C. Protect from light.
For research use only.
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