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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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LAG3 Antibody (aa70-99, clone 17B4, FITC) IHC-plus™ LS-B2237

Note: This antibody replaces LS-C18693, LS-C8409, LS-C8410, LS-C8407, LS-C8408
Anti-LAG-3 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

LAG3 Antibody (aa70-99, clone 17B4, FITC) IHC-plus™ LS-B2237

Note: This antibody replaces LS-C18693, LS-C8409, LS-C8410, LS-C8407, LS-C8408
Antibody:
Mouse Monoclonal to Human LAG3
Application:
IHC, IHC-P, Flo
Reactivity:
Human
Format:
FITC, Unmodified
Price
Catalog Number
$425 
LS-B2237-50
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human LAG3
Application:
IHC, IHC-P, Flo
Reactivity:
Human
Format:
FITC, Unmodified

Specifications

Description
LAG3 antibody LS-B2237 is an FITC-conjugated mouse monoclonal antibody to human LAG3. Validated for Flow and IHC. Tested on 20 paraffin-embedded human tissues. Cited in 4 publications.

Target
Human LAG3
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
17B4
Conjugations
FITC
Purification
Purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Flow Cytometry (1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
LAG3 antibody was raised against synthetic peptide corresponding to 30 aa (GPPAAAPGHPLAPGPHPAAPSSWGPRPRRY) from the first N-terminal D1 domain of human LAG-3 (lymphocyte activation gene-3). Percent identity by BLAST analysis: Human (100%); Gorilla (93%); Gibbon (90%); Monkey (83%).
Epitope
aa70-99
Specificity
Recognizes the 30 aa extra-loop of the first N-terminal D1 domain of human LAG-3.
Usage
Immunohistochemistry: LS-B2237 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2237 was determined to be 10 ug/ml. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS containing 50mM Tris-HCl, 1% BSA, 0.02% sodium azide.
Storage
Store at 4°C. Protect from light.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About LAG3
P18627 NM_002286 P18627

Validation

Anti-LAG-3 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
IHC Validated
Anti-LAG-3 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-LAG-3 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Publications (4)

Liver environment and HCV replication affect human T-cell phenotype and expression of inhibitory receptors. Kroy DC, Ciuffreda D, Cooperrider JH, Tomlinson M, Hauck GD, Aneja J, Berger C, Wolski D, Carrington M, Wherry EJ, Chung RT, Tanabe KK, Elias N, Freeman GJ, de Kruyff RH, Misdraji J, Kim AY, Lauer GM. Gastroenterology. 2014 Feb;146:550-61.

LAG3 expression in active Mycobacterium tuberculosis infections. Phillips BL, Mehra S, Ahsan MH, Selman M, Khader SA, Kaushal D. The American journal of pathology. 2015 185:820-33. (IHC; Macaque)

Immunological biomarkers predict HIV-1 viral rebound after treatment interruption. Hurst J, Hoffmann M, Pace M, Williams JP, Thornhill J, Hamlyn E, Meyerowitz J, Willberg C, Koelsch KK, Robinson N, Brown H, Fisher M, Kinloch S, Cooper DA, Schechter M, Tambussi G, Fidler S, Babiker A, Weber J, Kelleher AD, Phillips RE, Frater J. Nature communications. 2015 6:8495. (Flo; Human)

Exhaustion of Activated CD8 T Cells Predicts Disease Progression in Primary HIV-1 Infection. Hoffmann M, Pantazis N, Martin GE, Hickling S, Hurst J, Meyerowitz J, Willberg CB, Robinson N, Brown H, Fisher M, Kinloch S, Babiker A, Weber J, Nwokolo N, Fox J, Fidler S, Phillips R, Frater J,; SPARTAC and CHERUB Investigators. PLoS pathogens. 2016 12:e1005661. (Flo; Human)

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Requested From: United States
Date Requested: 7/19/2019
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