Target
Human KRT19 / CK19 / Cytokeratin 19
Synonyms
KRT19 | CK19 | Cytokeratin 19 | Cytokeratin-19 | Keratin 19 | Keratin-19 | CK-19 | K19 | K1CS | Keratin, type I, 40-kd
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Immunogen
Cytokeratin 19.
Specificity
Recognizes cytokeratin 19 from glandular-type epithelia and most carcinomas. Does not recognize cytokeratin from hepatocytes and hepatocellular carcinoma.
Applications
- IHC
- IHC - Paraffin
- IHC - Frozen
- ICC
- Western blot (1:100 - 1:1000)
- Flow Cytometry (1:100 - 1:200)
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Usage
Suitable for use in Immunohistochemistry, Immunocytochemistry, Flow Cytometry and Western Blot. Immunohistochemistry: 1:100-1:200 using avidin-biotinylated HRP complex (ABC) (Frozen and paraffin sections). Note: Paraffin tissues require pretreatment with pepsin or trypsin. Flow Cytometry: 1:100-1:200. Western Blot: 1:100-1:1000. Optimum dilutions to be determined by researcher. Hybridoma: Hybridization of SP2/0 mouse myeloma cells with spleen cells from Balb/c mice immunized with a cytoskeletal preparation of human bladder carcinoma cell line T24.
Presentation
PBS, pH 7.2, 0.1% Sodium Azide, No stabilizing proteins added
Storage
Store at 4°C. Avoid freezing.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About KRT19 / CK19 / Cytokeratin 19