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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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KPNA3 / Importin Alpha 4 Antibody (C Terminus, Biotin) LS‑C742491
Importin Alpha 4 antibody LS-C742491 is a biotin-conjugated goat polyclonal antibody to Importin Alpha 4 (KPNA3) from human, mouse and rat. Validated for ICC, IF, IHC, IP, Peptide-ELISA and WB.
Catalog
Size
Price
LS-C742491-100
100 µg
$405

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Antibody
Species: Human
Applications: Western blot

Product Description

Importin Alpha 4 antibody LS-C742491 is a biotin-conjugated goat polyclonal antibody to Importin Alpha 4 (KPNA3) from human, mouse and rat. Validated for ICC, IF, IHC, IP, Peptide-ELISA and WB.
About KPNA3 / Importin Alpha 4
Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. O00505 NM_002267 NP_002258.2

KPNA3 Antibody, Importin alpha-3 Antibody, Importin alpha Q2 Antibody, Karyopherin subunit alpha-3 Antibody, Importin-alpha-Q2 Antibody, IPOA4 Antibody, Qip2 Antibody, SRP1 Antibody, HSRP1 Antibody, Importin alpha 4 Antibody, Importin subunit alpha-3 Antibody, SRP1-gamma Antibody, SRP1gamma Antibody, SRP4 Antibody


Specifications

Target
Human KPNA3 / Importin Alpha 4
Host
Goat
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Predicted
Dog, Cow (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Biotin
Purification
ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
Modifications
Unmodified
Applications
  • IHC
  • ICC
  • Immunofluorescence
  • Western blot
  • Immunoprecipitation
  • Peptide Enzyme-Linked Immunosorbent Assay
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CDPTANLQTKEFNF
Epitope
C Terminus
Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 55kDa band observed in Rat Testis lysates (calculated MW of 57.8kDa according to Human NP_002258.2 and Rat NP_001014792.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 1-3 ug/ml.
Presentation
Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images

Western blot

Biotinylated antibody (1µg/ml) staining of Rat Testis lysate (35µg protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker
Biotinylated antibody (1µg/ml) staining of Rat Testis lysate (35µg protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker

Western blot

Biotinylated antibody (1µg/ml) staining of Rat Testis lysate (35µg protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker
Biotinylated antibody (1µg/ml) staining of Rat Testis lysate (35µg protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker

Western blot

Biotinylated antibody (1µg/ml) staining of Rat Testis lysate (35µg protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker
Biotinylated antibody (1µg/ml) staining of Rat Testis lysate (35µg protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker

Western blot

Biotinylated antibody (1µg/ml) staining of Rat Testis lysate (35µg protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker
Biotinylated antibody (1µg/ml) staining of Rat Testis lysate (35µg protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker

Requested From: United States
Date Requested: 7/22/2018

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