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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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KPNA3 / Importin Alpha 4 Antibody (aa509‑521) IHC‑plus™ LS‑B2289
Note: This antibody replaces LS-C55043, LS-C71263
Importin Alpha 4 antibody LS-B2289 is an unconjugated goat polyclonal antibody to Importin Alpha 4 (KPNA3) from human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
Catalog
Size
Price
LS-B2289-50
50 µg (0.5 mg/ml)
$425

Popular KPNA3 / Importin Alpha 4 Products

Anti-KPNA3 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5341 concentration 5 ug/ml.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
Antibody
Species: Human
Applications: Western blot
Detection of Human KPNA3 by Immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-KPNA3 used at a dilution of 1:200 (1 Detection: DAB.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, Proximity Ligation Assay
Biotinylated antibody (1µg/ml) staining of Rat Testis lysate (35µg protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker
Species: Human, Mouse, Rat
Applications: IHC, ICC, Immunofluorescence, Western blot, Immunoprecipitation, Peptide Enzyme-Linked Immunosorbent Assay

Product Description

Importin Alpha 4 antibody LS-B2289 is an unconjugated goat polyclonal antibody to Importin Alpha 4 (KPNA3) from human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
About KPNA3 / Importin Alpha 4
Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. O00505 NM_002267 NP_002258.2

KPNA3 Antibody, Importin alpha-3 Antibody, Importin alpha Q2 Antibody, Karyopherin subunit alpha-3 Antibody, Importin-alpha-Q2 Antibody, IPOA4 Antibody, Qip2 Antibody, SRP1 Antibody, HSRP1 Antibody, Importin alpha 4 Antibody, Importin subunit alpha-3 Antibody, SRP1-gamma Antibody, SRP1gamma Antibody, SRP4 Antibody


Specifications

Target
Human KPNA3 / Importin Alpha 4
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Chicken (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide C-DPTANLQTKEFNF from the C-terminus of human KPNA3 (NP_002258.2). Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Bat, Hamster, Elephant, Panda, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus, Lizard (100%); Xenopus (85%).
Blocking Peptide
LS-E25665 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B2289. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa509-521
Specificity
Human KPNA3.
Usage
Immunohistochemistry: LS-B2289 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2289 was determined to be 5 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.

Publications (2)

1
Notch1 signaling is mediated by importins alpha 3, 4, and 7. Huenniger K, Krmer A, Soom M, Chang I, Khler M, Depping R, Kehlenbach RH, Kaether C. Cellular and molecular life sciences : CMLS. 2010 67:3187-96. [PubMed:20454918] [PMC:PMC3128743]
2
Axotomy induces axonogenesis in hippocampal neurons by a mechanism dependent on importin . Ohara R, Hata K, Yasuhara N, Mehmood R, Yoneda Y, Nakagawa M, Yamashita T. Biochemical and biophysical research communications. 2011 405:697-702. (ICC; Rat) [PubMed:21291862]

Customer Reviews (0)


Images

Immunohistochemistry

Anti-KPNA3 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2289 concentration 5 ug/ml.
Anti-KPNA3 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2289 concentration 5 ug/ml.

Western blot

Antibody staining (0.1 ug/ml) of Human Testis lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Antibody staining (0.1 ug/ml) of Human Testis lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Immunohistochemistry

Anti-KPNA3 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2289 concentration 5 ug/ml.
Anti-KPNA3 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2289 concentration 5 ug/ml.

Western blot

Antibody staining (0.1 ug/ml) of Human Testis lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Antibody staining (0.1 ug/ml) of Human Testis lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Immunohistochemistry

Anti-KPNA3 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2289 concentration 5 ug/ml.
Anti-KPNA3 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2289 concentration 5 ug/ml.

Western blot

Antibody staining (0.1 ug/ml) of Human Testis lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Antibody staining (0.1 ug/ml) of Human Testis lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Immunohistochemistry

Anti-KPNA3 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2289 concentration 5 ug/ml.
Anti-KPNA3 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2289 concentration 5 ug/ml.

Western blot

Antibody staining (0.1 ug/ml) of Human Testis lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Antibody staining (0.1 ug/ml) of Human Testis lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Requested From: United States
Date Requested: 9/19/2018

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