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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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KLF4 Antibody (N‑Terminus) LS‑C357947
KLF4 antibody LS-C357947 is an unconjugated rabbit polyclonal antibody to KLF4 from human, mouse and rat. Validated for WB.
Catalog
Size
Price
LS-C357947-25
25 µg
$255
LS-C357947-100
100 µg
$355
Description
KLF4 antibody LS-C357947 is an unconjugated rabbit polyclonal antibody to KLF4 from human, mouse and rat. Validated for WB.
Target
Human KLF4
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot (1 - 3 µg/ml)
Immunogen
KLF4 antibody was raised against a recombinant protein fragment from N-terminal human KLF4.
Epitope
N-Terminus
Specificity
Human KLF4
Usage
Krueppel-like factor family members are involved in growth regulation and a wide range of developmental processes. KLF4 is highly expressed in epithelial tissues including skin and stomach/intestine. Studies have shown that KLF4 can function as both an oncogene, upregulating hSSC, and a tumor suppressor, down-regulating colorectal cancer. KLF4 is involved in the differentiation of epithelial cells and may also function in skeletal and kidney development.
Presentation
PBS, pH 7.4, 0.1 mg/ml BSA, 0.05% Sodium Azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About KLF4
O43474 NM_004235 NP_004226.3

Popular KLF4 Products

Anti-KLF4 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Anti-KLF4 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Sheep, Chicken, Xenopus, Zebrafish, Drosophila
Applications: IHC, IHC - Paraffin, Western blot
Immunofluorescence of KLF4 antibody (5 ug/ml). HeLa cells transfected with pMX constructs of human KLF4 (top) and NIH3T3 cells transfected with pMX constructs of mouse KLF4 (bottom) were analyzed at approximately 62 hours after transfection.
Species: Human
Applications: Immunofluorescence, Western blot, Flow Cytometry
Anti-KLF4 antibody IHC of human melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Bovine, Dog, Horse, Pig, Sheep
Applications: IHC, IHC - Paraffin, Western blot
Antibody (0.1 ug/ml) staining of Human Duodenum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay

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Images

Western blot

KLF4 antibody western blot of mouse samples
KLF4 antibody western blot of mouse samples

Western blot

KLF4 antibody western blot of human samples
KLF4 antibody western blot of human samples

Western blot

KLF4 antibody western blot of mouse samples
KLF4 antibody western blot of mouse samples

Western blot

KLF4 antibody western blot of human samples
KLF4 antibody western blot of human samples

Western blot

KLF4 antibody western blot of mouse samples
KLF4 antibody western blot of mouse samples

Western blot

KLF4 antibody western blot of human samples
KLF4 antibody western blot of human samples

Western blot

KLF4 antibody western blot of mouse samples
KLF4 antibody western blot of mouse samples

Western blot

KLF4 antibody western blot of human samples
KLF4 antibody western blot of human samples

Requested From: United States
Date Requested: 11/18/2018
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