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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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KLF4 Antibody (aa187‑474, APC) LS‑C716640
KLF4 antibody LS-C716640 is an APC-conjugated rabbit polyclonal antibody to KLF4 from human and mouse. Validated for IHC and WB.
Catalog
Size
Price
LS-C716640-20
20 µl
$225
LS-C716640-100
100 µl
$295
LS-C716640-200
200 µl
$355
LS-C716640-1
1 ml
$740
LS-C716640-10
10 ml
$5,580

Popular KLF4 Products

Anti-KLF4 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Anti-KLF4 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Sheep, Chicken, Xenopus, Zebrafish, Drosophila
Applications: IHC, IHC - Paraffin, Western blot
Immunofluorescence of KLF4 antibody (5 ug/ml). HeLa cells transfected with pMX constructs of human KLF4 (top) and NIH3T3 cells transfected with pMX constructs of mouse KLF4 (bottom) were analyzed at approximately 62 hours after transfection.
Species: Human
Applications: Immunofluorescence, Western blot, Flow Cytometry
Anti-KLF4 antibody IHC of human melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Bovine, Dog, Horse, Pig, Sheep
Applications: IHC, IHC - Paraffin, Western blot
Antibody (0.1 ug/ml) staining of Human Duodenum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay

Product Description

KLF4 antibody LS-C716640 is an APC-conjugated rabbit polyclonal antibody to KLF4 from human and mouse. Validated for IHC and WB.

Specifications

Target
Human KLF4
Synonyms
KLF4, EZF, GKLF, Kruppel-like factor 4 (gut), Hezf, Krueppel-like factor 4
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Allophycocyanin. Also available Unconjugated or conjugated with Biotin, FITC, Biotin, FITC, Cy3, HRP, PE, APC, Cy7.
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Modifications
Unmodified
Applications
  • IHC
  • Western blot
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
KLF4 antibody was raised against recombinant KLF4 (Leu187-His474) expressed in E. coli.
Epitope
aa187-474
Specificity
The antibody is a rabbit polyclonal antibody raised against KLF4. It has been selected for its ability to recognize KLF4 in immunohistochemical staining and western blotting.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Avoid repeated freeze/thaw cycles. Store at 4°C for frequent use. Aliquot and store at -20°C for 12 months.
Restrictions
For research use only.
About KLF4
Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. O43474 NM_004235 NP_004226.3


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Images

Western blot

Western blot of recombinant KLF4.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant KLF4. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot of recombinant KLF4.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant KLF4. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot of recombinant KLF4.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant KLF4. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot of recombinant KLF4.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant KLF4. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 11/12/2018

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