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Catalog Number Size Price
LS-C745344-25 25 µl (1.1 mg/ml) $275 
KIAA0652 / ATG13 Antibody - Western blot using the affinity purified anti-ATG13 pS318 antibody shows detection of phosphorylated ATG13 in 293T cells engineered to coexpress Ulk1 and Atg13 (Ulk1 + Atg13). In the left lane was loaded kinase-dead hypophosphorylated Ulk1-K46A mutant + ATG13. The right lane contains the 293T Ulk1 + ATG13 lysate and shows detection at approximately 57 kDa. The antibody was purified and resolved by SDS-PAGE, then transferred to nitrocellulose membrane. The membrane was blocked with 5% Blotto and probed with the primary antibody at 1µg/mL overnight at 4°C. After washing, the membrane was probed with Goat Anti-Rabbit HRP secondary 1:5000 in detection buffer for 45 minutes at room temperature.

Polyclonal Rabbit anti‑Human KIAA0652 / ATG13 Antibody (phospho‑Ser318, WB) LS‑C745344

Polyclonal Rabbit anti‑Human KIAA0652 / ATG13 Antibody (phospho‑Ser318, WB) LS‑C745344

Antibody:
KIAA0652 / ATG13 Rabbit anti-Human Polyclonal (pSer318) Antibody
Application:
WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C745344-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
KIAA0652 / ATG13 Rabbit anti-Human Polyclonal (pSer318) Antibody
Application:
WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
ATG13 antibody LS-C745344 is an unconjugated rabbit polyclonal antibody to human ATG13 (KIAA0652) (pSer318). Validated for ELISA, Flow and WB.
Target
Human KIAA0652 / ATG13
Synonyms
ATG13 | Autophagy-related protein 13 | KIAA0652 | Autophagy related 13
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Anti-ATG13 pS318 antibody was prepared by repeated immunizations with a synthetic peptide corresponding to the region near S318 of ATG13.
Epitope
pSer318
Specificity
Reactivity occurs against human ATG13 pS318 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human ATG13 is minimal by ELISA and western blot. A BLAST analysis was used to suggest cross reactivity with ATG13 from human based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.
Applications
  • Western blot (1:1000)
  • Flow Cytometry
  • ELISA (1:25000 - 1:175000)
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About KIAA0652 / ATG13
O75143 NM_014741 NP_001136145.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/11/2021