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Catalog Number Size Price
LS-B4967-50 50 µg (0.5 mg/ml) $485 
KIAA0517 / TRIM2 Antibody - Anti-TRIM2 / RNF86 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 6.25 ug/ml.
KIAA0517 / TRIM2 Antibody - KIAA0517 / TRIM2 antibody (0.5µg/ml) staining of Rat Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
KIAA0517 / TRIM2 Antibody - TRIM2 antibody (0.5 ug/ml) staining of Rat Brain lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
KIAA0517 / TRIM2 Antibody - TRIM2 Antibody Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
KIAA0517 / TRIM2 Antibody - Anti-TRIM2 / RNF86 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 6.25 ug/ml.
KIAA0517 / TRIM2 Antibody - KIAA0517 / TRIM2 antibody (0.5µg/ml) staining of Rat Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
KIAA0517 / TRIM2 Antibody - TRIM2 antibody (0.5 ug/ml) staining of Rat Brain lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
KIAA0517 / TRIM2 Antibody - TRIM2 Antibody Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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IHC‑plus™ Polyclonal Goat anti‑Human KIAA0517 / TRIM2 Antibody (aa758‑771, IHC, WB) LS‑B4967

IHC‑plus™ Polyclonal Goat anti‑Human KIAA0517 / TRIM2 Antibody (aa758‑771, IHC, WB) LS‑B4967

Note: This antibody replaces LS-C55620
Antibody:
KIAA0517 / TRIM2 Goat anti-Human Polyclonal (aa758-771) Antibody
Application:
IHC, IHC-P, WB, Flo, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit, Chicken, Xenopus, Zebrafish
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-B4967-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
KIAA0517 / TRIM2 Goat anti-Human Polyclonal (aa758-771) Antibody
Application:
IHC, IHC-P, WB, Flo, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit, Chicken, Xenopus, Zebrafish
Format:
Unconjugated, Unmodified

Specifications

Description
TRIM2 antibody LS-B4967 is an unconjugated goat polyclonal antibody to TRIM2 (KIAA0517) (aa758-771) from human. It is reactive with human, mouse, rat and other species. Validated for Flow, IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human KIAA0517 / TRIM2
Synonyms
TRIM2 | KIAA0517 | RNF86 | RING finger protein 86 | Tripartite motif containing 2 | Tripartite motif protein 2 | Tripartite motif protein TRIM2 | Tripartite motif-containing 2
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit, Chicken, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence DSGNHCFKVYRYLQ, from the C-Terminus of protein sequence according to NP_056086.2NP_001123539.1NP_001337983.1NP_001337984.1NP_001337985.1lNP_001337986.1.
Epitope
aa758-771
Specificity
Human TRIM2 / RNF86. This antibody is expected to recognise both reported isoforms (NP_056086.2; NP_001123539.1). Cross-reactivity with TRIM3 has been demonstrated.
Applications
  • IHC
  • IHC - Paraffin (6.25 µg/ml)
  • Western blot (0.5 - 1 µg/ml)
  • Flow Cytometry (10 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 80+85 kDa bands observed in Rat Brain lysates (calculated MW of 81.5 kDa according to Human NP_001123539.1, 84.4 accorindg to Human NP_056086.2 and 83.3 according to Rat NP_001102022.1). Recommended concentration: 0.5-2 ug/ml.. An additional band of unknown identity was also consistently observed at 30 kDa. This band was successfully blocked by incubation with the immunizing peptide.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About KIAA0517 / TRIM2
Q9C040 NM_015271 NP_056086.2

Validation

KIAA0517 / TRIM2 Antibody - Anti-TRIM2 / RNF86 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 6.25 ug/ml.
Anti-TRIM2 / RNF86 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 6.25 ug/ml.
Anti-TRIM2 / RNF86 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 6.25 ug/ml.

LSBio Ratings

IHC-plus™ KIAA0517 / TRIM2 Antibody (aa758-771) for IHC, WB/Western, Flow LS-B4967 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/18/2024