Menu
Products
Services
Research Areas
Resources
Contact Us
Distributors
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Services
Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
Contact Us
LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121
Phone:
866-819-4732 (Toll Free North America
206-374-1102 (International)
Fax:
866-206-6909 (Toll Free North America)
206-577-4565 (International)
How To Buy - Details about how to buy our products.
Orders@LSBio.com - To submit a new order.
Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, froforma invoice requests, or other billing issues.
Technical.Support@LSBio.com - To request technical information about an LSBio product or its applications
Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.
Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C156742-400 400 µl $325 
KIAA0226 / RUBICON Antibody - Formalin-fixed and paraffin-embedded human lymph with BARON Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
KIAA0226 / RUBICON Antibody - Fluorescent image of U251 cells stained with BARON antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated BARON primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). BARON immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
KIAA0226 / RUBICON Antibody - Western blot of BARON antibody in MCF-7 cell line lysates (35 ug/lane). BARON (arrow) was detected using the purified antibody.
KIAA0226 / RUBICON Antibody - Flow cytometric of MDA-231 cells using BARON Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
KIAA0226 / RUBICON Antibody - Formalin-fixed and paraffin-embedded human lymph with BARON Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
KIAA0226 / RUBICON Antibody - Fluorescent image of U251 cells stained with BARON antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated BARON primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). BARON immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
KIAA0226 / RUBICON Antibody - Western blot of BARON antibody in MCF-7 cell line lysates (35 ug/lane). BARON (arrow) was detected using the purified antibody.
KIAA0226 / RUBICON Antibody - Flow cytometric of MDA-231 cells using BARON Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
1 of 4
2 of 4
3 of 4
4 of 4

KIAA0226 / RUBICON Antibody (aa241‑271) LS‑C156742

KIAA0226 / RUBICON Antibody (aa241‑271) LS‑C156742

Antibody:
Rabbit Polyclonal to Human KIAA0226 / RUBICON
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$325 
LS-C156742-400
400 µl
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human KIAA0226 / RUBICON
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
RUBICON antibody LS-C156742 is an unconjugated rabbit polyclonal antibody to human RUBICON (KIAA0226 ). Validated for Flow, IF, IHC and WB.
Target
Human KIAA0226 / RUBICON
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:100)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E5779 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C156742. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa241-271
Specificity
This BARON antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 241-271 amino acids from the N-terminal region of human BARON.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About KIAA0226 / RUBICON
Q92622 D86979 BAA13215.3

Publications (0)

Customer Reviews (0)


Requested From: United States
Date Requested: 11/18/2019