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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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KCNJ5 / Kir3.4 / GIRK4 Antibody (N‑Terminus, HRP) LS‑C431651
GIRK4 antibody LS-C431651 is an HRP-conjugated rabbit polyclonal antibody to GIRK4 (KCNJ5 / Kir3.4) from human, bat, chimpanzee and other species. Validated for WB.
Catalog
Size
Price
LS-C431651-100
100 µl (0.65 mg/ml)
$385

Popular KCNJ5 / Kir3.4 / GIRK4 Products

Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Immunofluorescence analysis of A549 cells, using KCNJ5 Antibody. The picture on the right is blocked with the synthesized peptide.
Species: Human
Applications: Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Immunohistochemistry image at a dilution of 1:300 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human
Applications: Immunofluorescence, Western blot, ELISA

Product Description

GIRK4 antibody LS-C431651 is an HRP-conjugated rabbit polyclonal antibody to GIRK4 (KCNJ5 / Kir3.4) from human, bat, chimpanzee and other species. Validated for WB.

Specifications

Target
Human KCNJ5 / Kir3.4 / GIRK4
Synonyms
KCNJ5, CIR, GIRK-4, KATP1, Heart KATP channel, IRK-4, KATP-1, KIR3.4, LQT13, Cardiac inward rectifier, GIRK4
Host
Rabbit
Reactivity
Human, Chimpanzee, Bat, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Gibbon, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig, Chicken (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Horseradish Peroxidase. Also available Unconjugated or conjugated with Biotin, FITC.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
Synthetic peptide from N-Terminus of human KCNJ5 (P48544, NP_000881). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Marmoset, Elephant, Bat (100%); Gibbon, Monkey, Galago, Mouse, Rat, Hamster, Panda, Dog, Bovine, Horse, Pig, Opossum, Turkey, Chicken (92%); Rabbit (85%).
Epitope
N-Terminus
Specificity
Human KCNJ5 / Kir3.4
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
100 mM sodium phosphate, pH 7.6, 150 mM NaCl
Storage
Short term: store at 4°C. Long term: add glycerol to 40-50%, aliquot to avoid freeze-thaw cycles, and store at -20°C. Protect from light.
Restrictions
For research use only.
About KCNJ5 / Kir3.4 / GIRK4
This potassium channel is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. P48544 NM_000890 NP_000881.3


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Images

Western blot

KCNJ5 / Kir3.4 antibody Western blot of Fetal Liver lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
KCNJ5 / Kir3.4 antibody Western blot of Fetal Liver lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

KCNJ5 / Kir3.4 antibody Western blot of Fetal Liver lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
KCNJ5 / Kir3.4 antibody Western blot of Fetal Liver lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

KCNJ5 / Kir3.4 antibody Western blot of Fetal Liver lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
KCNJ5 / Kir3.4 antibody Western blot of Fetal Liver lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

KCNJ5 / Kir3.4 antibody Western blot of Fetal Liver lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
KCNJ5 / Kir3.4 antibody Western blot of Fetal Liver lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 11/14/2018

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