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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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KCNJ5 / Kir3.4 / GIRK4 Antibody LS-C670184

Immunohistochemistry image at a dilution of 1:300 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of A549 cells at a dilution of 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 °C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
Positive Western Blot detected in Mouse heart tissue. All lanes: KCNJ5 antibody at 4.85 µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 48 KDa. Observed band size: 48 KDa
Immunohistochemistry image at a dilution of 1:300 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of A549 cells at a dilution of 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 °C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
Positive Western Blot detected in Mouse heart tissue. All lanes: KCNJ5 antibody at 4.85 µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 48 KDa. Observed band size: 48 KDa
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KCNJ5 / Kir3.4 / GIRK4 Antibody LS-C670184

Antibody:
Rabbit Polyclonal to Human KCNJ5 / Kir3.4 / GIRK4
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$285 
LS-C670184-50
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human KCNJ5 / Kir3.4 / GIRK4
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
GIRK4 antibody LS-C670184 is an unconjugated rabbit polyclonal antibody to human GIRK4 (KCNJ5 / Kir3.4). Validated for ELISA, IF, IHC and WB.

Target
Human KCNJ5 / Kir3.4 / GIRK4
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, HRP, FITC.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Immunofluorescence
  • Western blot
  • ELISA
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Recombinant Human G protein-activated inward rectifier potassium channel 4 protein (348-419AA)
Presentation
0.01 M PBS, pH 7.4, 0.03% ProClin™ 300, 50% Glycerol
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About KCNJ5 / Kir3.4 / GIRK4
P48544 NM_000890 NP_000881.3

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Product
Species: Human
Applications: Flow Cytometry
Reactivity: Human
Range: 0.312-20 ng/ml
Reactivity: Human
Range: 3.12-200 ng/ml
Reactivity: Human
Range: 31.25-2000 pg/ml
Reactivity: Mouse
Range: 0.312-20 ng/ml

Requested From: United States
Date Requested: 5/26/2019
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