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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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KCNJ5 / Kir3.4 / GIRK4 Antibody (clone 8D2) IHC‑plus™ LS‑B13297 Best Seller
Note: This antibody replaces LS-C197573
GIRK4 antibody LS-B13297 is an unconjugated mouse monoclonal antibody to human GIRK4 (KCNJ5 / Kir3.4). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B13297-50
50 µg (0.5 mg/ml)
$485
Description
GIRK4 antibody LS-B13297 is an unconjugated mouse monoclonal antibody to human GIRK4 (KCNJ5 / Kir3.4). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human KCNJ5 / Kir3.4 / GIRK4
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal [8D2]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
KCNJ5 (NP_000881.3, aa321-419 partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human KCNJ5 / Kir3.4 / GIRK4
Usage
Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
About KCNJ5 / Kir3.4 / GIRK4
P48544 NM_000890 NP_000881.3

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Images

Immunohistochemistry - Paraffin

Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western blot

KCNJ5 monoclonal antibody (M01), clone 8D2. Western Blot analysis of KCNJ5 expression in K-562.
KCNJ5 monoclonal antibody (M01), clone 8D2. Western Blot analysis of KCNJ5 expression in K-562.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged KCNJ5 is 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged KCNJ5 is 0.03 ng/ml as a capture antibody.

Immunohistochemistry - Paraffin

Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western blot

KCNJ5 monoclonal antibody (M01), clone 8D2. Western Blot analysis of KCNJ5 expression in K-562.
KCNJ5 monoclonal antibody (M01), clone 8D2. Western Blot analysis of KCNJ5 expression in K-562.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged KCNJ5 is 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged KCNJ5 is 0.03 ng/ml as a capture antibody.

Immunohistochemistry - Paraffin

Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western blot

KCNJ5 monoclonal antibody (M01), clone 8D2. Western Blot analysis of KCNJ5 expression in K-562.
KCNJ5 monoclonal antibody (M01), clone 8D2. Western Blot analysis of KCNJ5 expression in K-562.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged KCNJ5 is 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged KCNJ5 is 0.03 ng/ml as a capture antibody.

Immunohistochemistry - Paraffin

Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western blot

KCNJ5 monoclonal antibody (M01), clone 8D2. Western Blot analysis of KCNJ5 expression in K-562.
KCNJ5 monoclonal antibody (M01), clone 8D2. Western Blot analysis of KCNJ5 expression in K-562.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged KCNJ5 is 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged KCNJ5 is 0.03 ng/ml as a capture antibody.

Requested From: United States
Date Requested: 11/19/2018
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