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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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KARS Antibody (clone 8G12C1) IHC‑plus™ LS‑B4096 Best Seller
Note: This antibody replaces LS-C108874
KARS antibody LS-B4096 is an unconjugated mouse monoclonal antibody to human KARS. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B4096-50
50 µl
$425

Popular KARS Products

Detection of Human KARS by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-KARS used at a dilution of 1:200 (1 Detection: DAB.
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation
Anti-KARS antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8077 dilution 5-10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Guinea pig, Horse, Pig, Rabbit
Applications: IHC, IHC - Paraffin, Western blot
Detection of Human KARS by Immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-KARS used at a dilution of 1:250. Epitope Retrieval Buffer-High pH (IHC-101J) was substituted for Epitope Retrieval Buffer-Reduced pH.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence
Immunohistochemistry of Human colon cancer using KARS Polyclonal Antibody at dilution of 1:60.
Species: Human, Mouse
Applications: IHC, Western blot, ELISA

Product Description

KARS antibody LS-B4096 is an unconjugated mouse monoclonal antibody to human KARS. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About KARS
Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of diadenosine oligophosphate (Ap4A), a signaling molecule involved in the activation of MITF transcriptional activity. Q15046 NM_005548 NP_005539.1

KARS Antibody, CMTRIB Antibody, KARS1 Antibody, KARS2 Antibody, KRS Antibody, Lysine--tRNA ligase Antibody, LysRS Antibody, KIAA0070 Antibody, Lysine tRNA ligase Antibody, Lysyl-tRNA synthetase Antibody


Specifications

Target
Human KARS
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [8G12C1]
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:200)
  • Western blot (1:100 - 1:500)
  • ELISA (1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
KARS antibody was raised against purified recombinant fragment of KARS(aa90-174) expressed in E. Coli.\
Specificity
Human
Presentation
Ascitic fluid containing 0.03% sodium azide
Storage
Short term: 4°C; Long term: -20°C.
Restrictions
For research use only.

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Images

Immunohistochemistry

Anti-KARS antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4096 dilution 1:200.
Anti-KARS antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4096 dilution 1:200.

Immunohistochemistry

Anti-KARS antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4096 dilution 1:200.
Anti-KARS antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4096 dilution 1:200.

Western blot

Western blot using KARS mouse monoclonal antibody against truncated Trx-KARS recombinant protein (1), truncated MBP-KARS (aa90-174) and full length KARS (aa1-188) transfected CHO-K1 cell lysate (3).
Western blot using KARS mouse monoclonal antibody against truncated Trx-KARS recombinant protein (1), truncated MBP-KARS (aa90-174) and full length KARS (aa1-188) transfected CHO-K1 cell lysate (3).

Immunohistochemistry

Anti-KARS antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4096 dilution 1:200.
Anti-KARS antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4096 dilution 1:200.

Immunohistochemistry

Anti-KARS antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4096 dilution 1:200.
Anti-KARS antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4096 dilution 1:200.

Western blot

Western blot using KARS mouse monoclonal antibody against truncated Trx-KARS recombinant protein (1), truncated MBP-KARS (aa90-174) and full length KARS (aa1-188) transfected CHO-K1 cell lysate (3).
Western blot using KARS mouse monoclonal antibody against truncated Trx-KARS recombinant protein (1), truncated MBP-KARS (aa90-174) and full length KARS (aa1-188) transfected CHO-K1 cell lysate (3).

Immunohistochemistry

Anti-KARS antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4096 dilution 1:200.
Anti-KARS antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4096 dilution 1:200.

Immunohistochemistry

Anti-KARS antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4096 dilution 1:200.
Anti-KARS antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4096 dilution 1:200.

Western blot

Western blot using KARS mouse monoclonal antibody against truncated Trx-KARS recombinant protein (1), truncated MBP-KARS (aa90-174) and full length KARS (aa1-188) transfected CHO-K1 cell lysate (3).
Western blot using KARS mouse monoclonal antibody against truncated Trx-KARS recombinant protein (1), truncated MBP-KARS (aa90-174) and full length KARS (aa1-188) transfected CHO-K1 cell lysate (3).

Immunohistochemistry

Anti-KARS antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4096 dilution 1:200.
Anti-KARS antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4096 dilution 1:200.

Immunohistochemistry

Anti-KARS antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4096 dilution 1:200.
Anti-KARS antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4096 dilution 1:200.

Western blot

Western blot using KARS mouse monoclonal antibody against truncated Trx-KARS recombinant protein (1), truncated MBP-KARS (aa90-174) and full length KARS (aa1-188) transfected CHO-K1 cell lysate (3).
Western blot using KARS mouse monoclonal antibody against truncated Trx-KARS recombinant protein (1), truncated MBP-KARS (aa90-174) and full length KARS (aa1-188) transfected CHO-K1 cell lysate (3).

Requested From: United States
Date Requested: 9/21/2018

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