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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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JUNB / JUN‑B Antibody (AP) LS‑C509318
JUN-B antibody LS-C509318 is an AP-conjugated rabbit polyclonal antibody to human JUN-B (JUNB). Validated for ICC and WB.
Catalog
Size
Price
LS-C509318-100
100 µl
$800
Description
JUN-B antibody LS-C509318 is an AP-conjugated rabbit polyclonal antibody to human JUN-B (JUNB). Validated for ICC and WB.
Target
Human JUNB / JUN-B
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Alkaline Phosphatase. Also available Unconjugated or conjugated with APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • ICC
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
JUNB / JUN-B antibody was raised against linear peptide corresponding to human JunB (KLH).
Specificity
Recognizes human endogenous JunB, and may also detect various homodimeric or heterodimeric states. Species sequence homology: mouse, rat, dog, horse, cow.
Presentation
PBS, pH 7.2, No preservatives added
Storage
Store at 4°C.
Restrictions
For research use only.
About JUNB / JUN-B
P17275 NM_002229 NP_002220.1

Popular JUNB / JUN-B Products

Immunofluorescence analysis of JunB was done on 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with JunB Rabbit Polyclonal Antibody at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d is a merged image showing nuclear localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
Species: Mouse, Hamster
Applications: Immunofluorescence, Western blot, Immunoprecipitation, Chromatin Immunoprecipitation
Western blot of Phospho-Jun B (S259) antibody
Species: Human, Mouse, Rat
Applications: IHC, Western blot, Immunoprecipitation, ELISA
Anti-JUNB antibody IHC of human uterus, endometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation, Peptide Enzyme-Linked Immunosorbent Assay
Immunohistochemical analysis of JUNB staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Mouse, Rat, Bovine, Sheep
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation
Antibody
Species: Human
Applications: Western blot

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Requested From: United States
Date Requested: 1/18/2019
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